Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

Fenfen Zhang; Xiaoli Wang; Shiyun Ai; Zhengdong Sun; Qiao Wan; Ziqiang Zhu; Yuezhong Xian; Litong Jin; Katsunobu Yamamoto

doi:10.1016/j.aca.2004.05.070

Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

Fenfen Zhang
, Xiaoli Wang
, Shiyun Ai
, Zhengdong Sun
, Qiao Wan
, Ziqiang Zhu
, Yuezhong Xian
, Litong Jin^*
, Katsunobu Yamamoto

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

345 Scopus citations

Abstract

A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85°C and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 × 10^-6 to 1.0 × 10^-3 mol L^-1 with a detection limit of 2.0 × 10^-6 mol L^-1 at 3σ. The apparent K_M^app value for the uric acid sensor was estimated to be 0.238 mM, showing a high affinity.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Analytica Chimica Acta
Volume	519
Issue number	2
DOIs	https://doi.org/10.1016/j.aca.2004.05.070
State	Published - 16 Aug 2004

Keywords

Reagentless biosensor
Uric acid
Uricase
ZnO nanorods

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10.1016/j.aca.2004.05.070

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title = "Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor",

abstract = "A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85°C and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 × 10-6 to 1.0 × 10-3 mol L-1 with a detection limit of 2.0 × 10-6 mol L-1 at 3σ. The apparent KMapp value for the uric acid sensor was estimated to be 0.238 mM, showing a high affinity.",

keywords = "Reagentless biosensor, Uric acid, Uricase, ZnO nanorods",

author = "Fenfen Zhang and Xiaoli Wang and Shiyun Ai and Zhengdong Sun and Qiao Wan and Ziqiang Zhu and Yuezhong Xian and Litong Jin and Katsunobu Yamamoto",

year = "2004",

month = aug,

day = "16",

doi = "10.1016/j.aca.2004.05.070",

language = "英语",

volume = "519",

pages = "155--160",

journal = "Analytica Chimica Acta",

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publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

AU - Zhang, Fenfen

AU - Wang, Xiaoli

AU - Ai, Shiyun

AU - Sun, Zhengdong

AU - Wan, Qiao

AU - Zhu, Ziqiang

AU - Xian, Yuezhong

AU - Jin, Litong

AU - Yamamoto, Katsunobu

PY - 2004/8/16

Y1 - 2004/8/16

N2 - A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85°C and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 × 10-6 to 1.0 × 10-3 mol L-1 with a detection limit of 2.0 × 10-6 mol L-1 at 3σ. The apparent KMapp value for the uric acid sensor was estimated to be 0.238 mM, showing a high affinity.

AB - A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85°C and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 × 10-6 to 1.0 × 10-3 mol L-1 with a detection limit of 2.0 × 10-6 mol L-1 at 3σ. The apparent KMapp value for the uric acid sensor was estimated to be 0.238 mM, showing a high affinity.

KW - Reagentless biosensor

KW - Uric acid

KW - Uricase

KW - ZnO nanorods

UR - https://www.scopus.com/pages/publications/3342986578

U2 - 10.1016/j.aca.2004.05.070

DO - 10.1016/j.aca.2004.05.070

M3 - 文章

AN - SCOPUS:3342986578

SN - 0003-2670

VL - 519

SP - 155

EP - 160

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2

ER -

Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

Abstract

Keywords

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