Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro

Zhi Feng Gan; Ji Sen Jiang; Yong Yang; Bing Du; Min Qian; Ping Zhang

doi:10.1002/jbm.a.31181

Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro

Zhi Feng Gan, Ji Sen Jiang^*, Yong Yang, Bing Du, Min Qian, Ping Zhang

^*Corresponding author for this work

School of Life Sciences

East China Normal University

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell. Homing peptide labeled with green fluorescent protein (A54-GFP) was successfully immobilized on the surfaces of magnetic nanoparticles and characterized by Fourier transform infrared spectroscopy as well as fluorescence microscopy. The binding efficiency was analyzed by performing adsorption equilibrium and SDS-PAGE electrophoresis. Specific binding of the nanoparticles functionalized with A54-GFP to human hepatocellular carcinoma cells in vitro was visualized using fluorescence microscopy. The results demonstrated the specificity of A54-GFP-coated magnetic nanoparticle to tumor cell, pointing to its great potential in magnetic cell separation and purification, magnetic resonance imaging (MRI), magnetic hyperthermia, and drug targeting.

Original language	English
Pages (from-to)	10-18
Number of pages	9
Journal	Journal of Biomedical Materials Research - Part A
Volume	84
Issue number	1
DOIs	https://doi.org/10.1002/jbm.a.31181
State	Published - 1 Jan 2008

Keywords

Homing peptide
Immobilization
In vitro
Magnetic nanoparticles
Specificity

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10.1002/jbm.a.31181

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abstract = "As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell. Homing peptide labeled with green fluorescent protein (A54-GFP) was successfully immobilized on the surfaces of magnetic nanoparticles and characterized by Fourier transform infrared spectroscopy as well as fluorescence microscopy. The binding efficiency was analyzed by performing adsorption equilibrium and SDS-PAGE electrophoresis. Specific binding of the nanoparticles functionalized with A54-GFP to human hepatocellular carcinoma cells in vitro was visualized using fluorescence microscopy. The results demonstrated the specificity of A54-GFP-coated magnetic nanoparticle to tumor cell, pointing to its great potential in magnetic cell separation and purification, magnetic resonance imaging (MRI), magnetic hyperthermia, and drug targeting.",

keywords = "Homing peptide, Immobilization, In vitro, Magnetic nanoparticles, Specificity",

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T1 - Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro

AU - Gan, Zhi Feng

AU - Jiang, Ji Sen

AU - Yang, Yong

AU - Du, Bing

AU - Qian, Min

AU - Zhang, Ping

PY - 2008/1/1

Y1 - 2008/1/1

N2 - As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell. Homing peptide labeled with green fluorescent protein (A54-GFP) was successfully immobilized on the surfaces of magnetic nanoparticles and characterized by Fourier transform infrared spectroscopy as well as fluorescence microscopy. The binding efficiency was analyzed by performing adsorption equilibrium and SDS-PAGE electrophoresis. Specific binding of the nanoparticles functionalized with A54-GFP to human hepatocellular carcinoma cells in vitro was visualized using fluorescence microscopy. The results demonstrated the specificity of A54-GFP-coated magnetic nanoparticle to tumor cell, pointing to its great potential in magnetic cell separation and purification, magnetic resonance imaging (MRI), magnetic hyperthermia, and drug targeting.

AB - As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell. Homing peptide labeled with green fluorescent protein (A54-GFP) was successfully immobilized on the surfaces of magnetic nanoparticles and characterized by Fourier transform infrared spectroscopy as well as fluorescence microscopy. The binding efficiency was analyzed by performing adsorption equilibrium and SDS-PAGE electrophoresis. Specific binding of the nanoparticles functionalized with A54-GFP to human hepatocellular carcinoma cells in vitro was visualized using fluorescence microscopy. The results demonstrated the specificity of A54-GFP-coated magnetic nanoparticle to tumor cell, pointing to its great potential in magnetic cell separation and purification, magnetic resonance imaging (MRI), magnetic hyperthermia, and drug targeting.

KW - Homing peptide

KW - Immobilization

KW - In vitro

KW - Magnetic nanoparticles

KW - Specificity

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AN - SCOPUS:36949018430

SN - 1549-3296

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JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

IS - 1

ER -

Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro

Abstract

Keywords

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