TY - JOUR
T1 - Immobilization of hemoglobin on stable mesoporous multilamellar silica vesicles and their activity and stability
AU - Zhu, Yufang
AU - Shen, Weihua
AU - Dong, Xiaoping
AU - Shi, Jianlin
PY - 2005/10
Y1 - 2005/10
N2 - A stable mesoporous multilamellar silica vesicle (MSV) was developed with a gallery pore size of about 14.0 nm. A simulative enzyme, hemoglobin (Hb), was immobilized on this newly developed MSV and a conventional mesoporous silica material SBA-15. The structures and the immobilization of Hb on the mesoporous supports were characterized with x-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier transform infrared, ultraviolet-visible spectroscopy, and so forth. MSV is a promising support for immobilizing Hb due to its large pore size and high Hb immobilization capacity (up to 522 mg/g) compared to SBA-15 (236 mg/g). Less than 5% Hb was leached from Hb/MSV at pH 6.0. The activity study indicated that the immobilized Hb retained most geroxidase activity compared to free Hb. Thermal stability of the immobilized Hb was improved by the proctetive environment of MSV and SBA-15. Such an Hb-meseporous support with high Hb immobilization capacity, high activity, and enhanced thermal stability will be attractive for practical application.
AB - A stable mesoporous multilamellar silica vesicle (MSV) was developed with a gallery pore size of about 14.0 nm. A simulative enzyme, hemoglobin (Hb), was immobilized on this newly developed MSV and a conventional mesoporous silica material SBA-15. The structures and the immobilization of Hb on the mesoporous supports were characterized with x-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier transform infrared, ultraviolet-visible spectroscopy, and so forth. MSV is a promising support for immobilizing Hb due to its large pore size and high Hb immobilization capacity (up to 522 mg/g) compared to SBA-15 (236 mg/g). Less than 5% Hb was leached from Hb/MSV at pH 6.0. The activity study indicated that the immobilized Hb retained most geroxidase activity compared to free Hb. Thermal stability of the immobilized Hb was improved by the proctetive environment of MSV and SBA-15. Such an Hb-meseporous support with high Hb immobilization capacity, high activity, and enhanced thermal stability will be attractive for practical application.
UR - https://www.scopus.com/pages/publications/29144492156
U2 - 10.1557/JMR.2005.0360
DO - 10.1557/JMR.2005.0360
M3 - 文章
AN - SCOPUS:29144492156
SN - 0884-2914
VL - 20
SP - 2682
EP - 2690
JO - Journal of Materials Research
JF - Journal of Materials Research
IS - 10
ER -