TY - JOUR
T1 - Identification of the Tibetan fox (Vulpes ferrilata) and the red fox (Vulpes vulpes) by copro-DNA diagnosis
AU - Jiang, Weibin
AU - Wang, Xiaoming
AU - Li, Min
AU - Wang, Zhenghuan
PY - 2011/1
Y1 - 2011/1
N2 - Studies on feeding habits based on faeces dissecting are imperative to understand the natural history of Tibetan foxes and their functions in the transmission of a lethal zoonotic parasite, Echinococcus multilocularis. However, Tibetan foxes and red foxes live sympatrically on the Tibetan plateau, China. Therefore, the faeces of Tibetan foxes must be distinguished from those of red foxes. We established a diagnostic method to distinguish the faeces of the two species by amplifying a portion of the mitochondrial cytochrome b gene (cytb) and digesting with the restriction enzymes BamHI and SspI, to produce specific diagnostic banding patterns. This PCR-RFLP assay enabled rapid, accurate and easily performed identification and differentiation of the two species.
AB - Studies on feeding habits based on faeces dissecting are imperative to understand the natural history of Tibetan foxes and their functions in the transmission of a lethal zoonotic parasite, Echinococcus multilocularis. However, Tibetan foxes and red foxes live sympatrically on the Tibetan plateau, China. Therefore, the faeces of Tibetan foxes must be distinguished from those of red foxes. We established a diagnostic method to distinguish the faeces of the two species by amplifying a portion of the mitochondrial cytochrome b gene (cytb) and digesting with the restriction enzymes BamHI and SspI, to produce specific diagnostic banding patterns. This PCR-RFLP assay enabled rapid, accurate and easily performed identification and differentiation of the two species.
KW - Non-invasive sampling
KW - PCR-RFLP
KW - Species identification
KW - Tibetan foxes
UR - https://www.scopus.com/pages/publications/78650093812
U2 - 10.1111/j.1755-0998.2010.02906.x
DO - 10.1111/j.1755-0998.2010.02906.x
M3 - 文章
C2 - 21429124
AN - SCOPUS:78650093812
SN - 1755-098X
VL - 11
SP - 206
EP - 210
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
IS - 1
ER -