Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu2+ in liver cells

Linlin Lu; Chongchong Feng; Jie Xu; Fengyang Wang; Haijun Yu; Zhiai Xu; Wen Zhang

doi:10.1016/j.bios.2017.01.066

Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu²⁺ in liver cells

Linlin Lu, Chongchong Feng, Jie Xu, Fengyang Wang, Haijun Yu, Zhiai Xu, Wen Zhang

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

Copper is closely related to liver damage, therefore, it is essential to develop a simple and sensitive strategy to detect copper ions (Cu²⁺) in liver cells. A hydrophobic carbon dots (HCDs)-based dual-emission fluorescent probe for Cu²⁺ was prepared by encapsulating HCDs in micelles formed by self-assembly of amphiphilic polymer DSPE-PEG and tetrakis (4-carboxyphenyl) porphyrin (TCPP)-modified DSPE-PEG. The obtained probe showed characteristic fluorescence emissions of HCDs and TCPP with large emission shift of 170 nm with single-wavelength excitation. In the presence of Cu²⁺, the fluorescence of TCPP was quenched and that of HCDs remained unchanged, displaying ratiometric fluorescence response to Cu²⁺. The developed probe exhibited high sensitivity (detection limit down to 36 nM) and selectivity to Cu²⁺ over other substances, and the probe was used to image the changes of Cu²⁺ level in liver cells successfully.

Original language	English
Pages (from-to)	101-108
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	92
DOIs	https://doi.org/10.1016/j.bios.2017.01.066
State	Published - 15 Jun 2017

Keywords

Copper ion
Fluorescence
Hydrophobic carbon dots
Micelle
Ratiometric
TCPP

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10.1016/j.bios.2017.01.066

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@article{16cab47201ab4ca59059bd1a0ff569a6,

title = "Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu2+ in liver cells",

abstract = "Copper is closely related to liver damage, therefore, it is essential to develop a simple and sensitive strategy to detect copper ions (Cu2+) in liver cells. A hydrophobic carbon dots (HCDs)-based dual-emission fluorescent probe for Cu2+ was prepared by encapsulating HCDs in micelles formed by self-assembly of amphiphilic polymer DSPE-PEG and tetrakis (4-carboxyphenyl) porphyrin (TCPP)-modified DSPE-PEG. The obtained probe showed characteristic fluorescence emissions of HCDs and TCPP with large emission shift of 170 nm with single-wavelength excitation. In the presence of Cu2+, the fluorescence of TCPP was quenched and that of HCDs remained unchanged, displaying ratiometric fluorescence response to Cu2+. The developed probe exhibited high sensitivity (detection limit down to 36 nM) and selectivity to Cu2+ over other substances, and the probe was used to image the changes of Cu2+ level in liver cells successfully.",

keywords = "Copper ion, Fluorescence, Hydrophobic carbon dots, Micelle, Ratiometric, TCPP",

author = "Linlin Lu and Chongchong Feng and Jie Xu and Fengyang Wang and Haijun Yu and Zhiai Xu and Wen Zhang",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

month = jun,

day = "15",

doi = "10.1016/j.bios.2017.01.066",

language = "英语",

volume = "92",

pages = "101--108",

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T1 - Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu2+ in liver cells

AU - Lu, Linlin

AU - Feng, Chongchong

AU - Xu, Jie

AU - Wang, Fengyang

AU - Yu, Haijun

AU - Xu, Zhiai

AU - Zhang, Wen

PY - 2017/6/15

Y1 - 2017/6/15

N2 - Copper is closely related to liver damage, therefore, it is essential to develop a simple and sensitive strategy to detect copper ions (Cu2+) in liver cells. A hydrophobic carbon dots (HCDs)-based dual-emission fluorescent probe for Cu2+ was prepared by encapsulating HCDs in micelles formed by self-assembly of amphiphilic polymer DSPE-PEG and tetrakis (4-carboxyphenyl) porphyrin (TCPP)-modified DSPE-PEG. The obtained probe showed characteristic fluorescence emissions of HCDs and TCPP with large emission shift of 170 nm with single-wavelength excitation. In the presence of Cu2+, the fluorescence of TCPP was quenched and that of HCDs remained unchanged, displaying ratiometric fluorescence response to Cu2+. The developed probe exhibited high sensitivity (detection limit down to 36 nM) and selectivity to Cu2+ over other substances, and the probe was used to image the changes of Cu2+ level in liver cells successfully.

AB - Copper is closely related to liver damage, therefore, it is essential to develop a simple and sensitive strategy to detect copper ions (Cu2+) in liver cells. A hydrophobic carbon dots (HCDs)-based dual-emission fluorescent probe for Cu2+ was prepared by encapsulating HCDs in micelles formed by self-assembly of amphiphilic polymer DSPE-PEG and tetrakis (4-carboxyphenyl) porphyrin (TCPP)-modified DSPE-PEG. The obtained probe showed characteristic fluorescence emissions of HCDs and TCPP with large emission shift of 170 nm with single-wavelength excitation. In the presence of Cu2+, the fluorescence of TCPP was quenched and that of HCDs remained unchanged, displaying ratiometric fluorescence response to Cu2+. The developed probe exhibited high sensitivity (detection limit down to 36 nM) and selectivity to Cu2+ over other substances, and the probe was used to image the changes of Cu2+ level in liver cells successfully.

KW - Copper ion

KW - Fluorescence

KW - Hydrophobic carbon dots

KW - Micelle

KW - Ratiometric

KW - TCPP

UR - https://www.scopus.com/pages/publications/85011975957

U2 - 10.1016/j.bios.2017.01.066

DO - 10.1016/j.bios.2017.01.066

M3 - 文章

C2 - 28193561

AN - SCOPUS:85011975957

SN - 0956-5663

VL - 92

SP - 101

EP - 108

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -

Hydrophobic-carbon-dot-based dual-emission micelle for ratiometric fluorescence biosensing and imaging of Cu²⁺ in liver cells

Abstract

Keywords

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