Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

Tiantian Chen; Liyan Yin; Chusen Huang; Yiqiao Qin; Weiping Zhu; Yufang Xu; Xuhong Qian

doi:10.1016/j.bios.2014.11.005

Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

Tiantian Chen
, Liyan Yin
, Chusen Huang^*
, Yiqiao Qin
, Weiping Zhu
, Yufang Xu
, Xuhong Qian

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu²⁺) in HEPES buffer (50mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu²⁺ ranging from 0-8.3μM (correlation coefficient R²=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R²=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity.

Original language	English
Pages (from-to)	259-265
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	66
DOIs	https://doi.org/10.1016/j.bios.2014.11.005
State	Published - 5 Apr 2015
Externally published	Yes

Keywords

Copper ion
Fluorescent probe
Histidine
Live cell imaging
Naphthalimide

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10.1016/j.bios.2014.11.005

Cite this

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title = "Highly selective {"}Off-On{"} fluorescent probe for histidine and its imaging in living cells",

abstract = "A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu2+) in HEPES buffer (50mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu2+ ranging from 0-8.3μM (correlation coefficient R2=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R2=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity.",

keywords = "Copper ion, Fluorescent probe, Histidine, Live cell imaging, Naphthalimide",

author = "Tiantian Chen and Liyan Yin and Chusen Huang and Yiqiao Qin and Weiping Zhu and Yufang Xu and Xuhong Qian",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

year = "2015",

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day = "5",

doi = "10.1016/j.bios.2014.11.005",

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TY - JOUR

T1 - Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

AU - Chen, Tiantian

AU - Yin, Liyan

AU - Huang, Chusen

AU - Qin, Yiqiao

AU - Zhu, Weiping

AU - Xu, Yufang

AU - Qian, Xuhong

PY - 2015/4/5

Y1 - 2015/4/5

N2 - A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu2+) in HEPES buffer (50mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu2+ ranging from 0-8.3μM (correlation coefficient R2=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R2=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity.

AB - A naphthalimide-based fluorescent probe CP has been synthesized with simple steps. It can selectively and sensitively recognize copper ions (Cu2+) in HEPES buffer (50mM, pH 7.2). The fluorescence intensity of CP is linearly proportional to the concentration of Cu2+ ranging from 0-8.3μM (correlation coefficient R2=0.9808). The resulted complex CP@Cu can serve as a turn-on fluorescent probe for the detection of histidine and histidine rich proteins in broad pH application range. Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R2=0.9912). Moreover, CP@Cu has potential for imaging histidine in vitro experiments and has promise in real sample applications with great validity.

KW - Copper ion

KW - Fluorescent probe

KW - Histidine

KW - Live cell imaging

KW - Naphthalimide

UR - https://www.scopus.com/pages/publications/84911945338

U2 - 10.1016/j.bios.2014.11.005

DO - 10.1016/j.bios.2014.11.005

M3 - 文章

C2 - 25437361

AN - SCOPUS:84911945338

SN - 0956-5663

VL - 66

SP - 259

EP - 265

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -

Highly selective "Off-On" fluorescent probe for histidine and its imaging in living cells

Abstract

Keywords

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