Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells

Chusen Huang; Qin Yin; Weiping Zhu; Yi Yang; Xin Wang; Xuhong Qian; Yufang Xu

doi:10.1002/anie.201101317

Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells

Chusen Huang^*
, Qin Yin
, Weiping Zhu
, Yi Yang
, Xin Wang
, Xuhong Qian
, Yufang Xu

^*Corresponding author for this work

East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

Original language	English
Pages (from-to)	7551-7556
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	33
DOIs	https://doi.org/10.1002/anie.201101317
State	Published - 8 Aug 2011
Externally published	Yes

Keywords

fluorescent probes
in situ imaging
protein labeling
redox proteomics
vicinal dithiols

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10.1002/anie.201101317

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abstract = "It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.",

keywords = "fluorescent probes, in situ imaging, protein labeling, redox proteomics, vicinal dithiols",

author = "Chusen Huang and Qin Yin and Weiping Zhu and Yi Yang and Xin Wang and Xuhong Qian and Yufang Xu",

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AU - Huang, Chusen

AU - Yin, Qin

AU - Zhu, Weiping

AU - Yang, Yi

AU - Wang, Xin

AU - Qian, Xuhong

AU - Xu, Yufang

PY - 2011/8/8

Y1 - 2011/8/8

N2 - It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

AB - It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function.

KW - fluorescent probes

KW - in situ imaging

KW - protein labeling

KW - redox proteomics

KW - vicinal dithiols

UR - https://www.scopus.com/pages/publications/79961242017

U2 - 10.1002/anie.201101317

DO - 10.1002/anie.201101317

M3 - 文章

C2 - 21721084

AN - SCOPUS:79961242017

SN - 1433-7851

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 33

ER -

Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells

Abstract

Keywords

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