High-quality covalently grafting hemoglobin on gold electrodes: Characterization, redox thermodynamics and bio-electrocatalysis

Yuan Tian; Qin Ran; Jingjing Xu; Yuezhong Xian; Ru Peng; Litong Jin

doi:10.1002/cphc.200900588

High-quality covalently grafting hemoglobin on gold electrodes: Characterization, redox thermodynamics and bio-electrocatalysis

Yuan Tian
, Qin Ran
, Jingjing Xu
, Yuezhong Xian^*
, Ru Peng
, Litong Jin

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Herein, we report a versatile surface chemistry methodology to covalently immobilize ligands and proteins to self-assembled monolayers (SAMs) on gold electrode. The strategy is based on two steps: 1) the coupling of soluble azido-PEG-amimo Iigand with an alkynyl-terminated monolayer via click reaction and 2) covalent immobilization hemoglobin (Hb) to the amine-terminated Iigand via carbodiimide reaction. Surface-enhanced Raman scattering spectroscopy (SERS), atomic force microsco-py (AFM), reflection absorption infrared spectroscopy (RAIR) and cyclic voltammetry are used to characterize the model interfacial reactions. We also demonstrate the excellent biocompatibility of the interface for Hb immobilization and reliable application of the proposed method for H₂O₂ biosensing. Moreover, the redox thermodynamics of the Fe³⁺/Fe²⁺couple in Hb is also investigated.

Original language	English
Pages (from-to)	3105-3111
Number of pages	7
Journal	ChemPhysChem
Volume	10
Issue number	17
DOIs	https://doi.org/10.1002/cphc.200900588
State	Published - 7 Dec 2009

Keywords

Biocompatibility
Carbodiimide reaction
Click chemistry
Electrochemistry
Surface chemistry

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10.1002/cphc.200900588

Cite this

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title = "High-quality covalently grafting hemoglobin on gold electrodes: Characterization, redox thermodynamics and bio-electrocatalysis",

abstract = "Herein, we report a versatile surface chemistry methodology to covalently immobilize ligands and proteins to self-assembled monolayers (SAMs) on gold electrode. The strategy is based on two steps: 1) the coupling of soluble azido-PEG-amimo Iigand with an alkynyl-terminated monolayer via click reaction and 2) covalent immobilization hemoglobin (Hb) to the amine-terminated Iigand via carbodiimide reaction. Surface-enhanced Raman scattering spectroscopy (SERS), atomic force microsco-py (AFM), reflection absorption infrared spectroscopy (RAIR) and cyclic voltammetry are used to characterize the model interfacial reactions. We also demonstrate the excellent biocompatibility of the interface for Hb immobilization and reliable application of the proposed method for H2O2 biosensing. Moreover, the redox thermodynamics of the Fe3+/Fe2+couple in Hb is also investigated.",

keywords = "Biocompatibility, Carbodiimide reaction, Click chemistry, Electrochemistry, Surface chemistry",

author = "Yuan Tian and Qin Ran and Jingjing Xu and Yuezhong Xian and Ru Peng and Litong Jin",

year = "2009",

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doi = "10.1002/cphc.200900588",

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T1 - High-quality covalently grafting hemoglobin on gold electrodes

T2 - Characterization, redox thermodynamics and bio-electrocatalysis

AU - Tian, Yuan

AU - Ran, Qin

AU - Xu, Jingjing

AU - Xian, Yuezhong

AU - Peng, Ru

AU - Jin, Litong

PY - 2009/12/7

Y1 - 2009/12/7

N2 - Herein, we report a versatile surface chemistry methodology to covalently immobilize ligands and proteins to self-assembled monolayers (SAMs) on gold electrode. The strategy is based on two steps: 1) the coupling of soluble azido-PEG-amimo Iigand with an alkynyl-terminated monolayer via click reaction and 2) covalent immobilization hemoglobin (Hb) to the amine-terminated Iigand via carbodiimide reaction. Surface-enhanced Raman scattering spectroscopy (SERS), atomic force microsco-py (AFM), reflection absorption infrared spectroscopy (RAIR) and cyclic voltammetry are used to characterize the model interfacial reactions. We also demonstrate the excellent biocompatibility of the interface for Hb immobilization and reliable application of the proposed method for H2O2 biosensing. Moreover, the redox thermodynamics of the Fe3+/Fe2+couple in Hb is also investigated.

AB - Herein, we report a versatile surface chemistry methodology to covalently immobilize ligands and proteins to self-assembled monolayers (SAMs) on gold electrode. The strategy is based on two steps: 1) the coupling of soluble azido-PEG-amimo Iigand with an alkynyl-terminated monolayer via click reaction and 2) covalent immobilization hemoglobin (Hb) to the amine-terminated Iigand via carbodiimide reaction. Surface-enhanced Raman scattering spectroscopy (SERS), atomic force microsco-py (AFM), reflection absorption infrared spectroscopy (RAIR) and cyclic voltammetry are used to characterize the model interfacial reactions. We also demonstrate the excellent biocompatibility of the interface for Hb immobilization and reliable application of the proposed method for H2O2 biosensing. Moreover, the redox thermodynamics of the Fe3+/Fe2+couple in Hb is also investigated.

KW - Biocompatibility

KW - Carbodiimide reaction

KW - Click chemistry

KW - Electrochemistry

KW - Surface chemistry

UR - https://www.scopus.com/pages/publications/73349111669

U2 - 10.1002/cphc.200900588

DO - 10.1002/cphc.200900588

M3 - 文章

AN - SCOPUS:73349111669

SN - 1439-4235

VL - 10

SP - 3105

EP - 3111

JO - ChemPhysChem

JF - ChemPhysChem

IS - 17

ER -

High-quality covalently grafting hemoglobin on gold electrodes: Characterization, redox thermodynamics and bio-electrocatalysis

Abstract

Keywords

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