GPR48-Induced keratinocyte proliferation occurs through HB-EGF mediated EGFR transactivation

Zhenlian Wang; Chang Jin; Hongxia Li; Canxia Li; Qiang Hou; Mingyao Liu; Xiang Da Eric Dong; Li Li Tu

doi:10.1016/j.febslet.2010.08.028

GPR48-Induced keratinocyte proliferation occurs through HB-EGF mediated EGFR transactivation

Zhenlian Wang
, Chang Jin
, Hongxia Li
, Canxia Li
, Qiang Hou
, Mingyao Liu
, Xiang Da Eric Dong
, Li Li Tu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

GPR48 can mediate keratinocyte proliferation and migration. Our investigations showed that AG1478, an inhibitor of EGFR tyrosine kinase, could block GPR48-mediated cellular processes. AG1478 treatment of Gpr48^+/+ cells also decreased phosphorylation of EGFR, ERK and STAT3. Subsequent screening using conditioned media immunodepleted of EGFR ligands identified HB-EGF as the ligand responsible for phosphorylation of EGFR, ERK and STAT3. HB-EGF was reduced in Gpr48^-/- cell culture medium, but its addition restored the phosphorylation of EGFR, ERK, STAT3, as well as cell proliferation. Confirmation that GPR48 mediates EGFR signaling pathway through HB-EGF was subsequently performed using an inhibitor of HB-EGF.

Original language	English
Pages (from-to)	4057-4062
Number of pages	6
Journal	FEBS Letters
Volume	584
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2010.08.028
State	Published - Sep 2010
Externally published	Yes

Keywords

Cell proliferation
EGFR
GPR48
HB-EGF
Keratinocyte

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10.1016/j.febslet.2010.08.028

Cite this

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title = "GPR48-Induced keratinocyte proliferation occurs through HB-EGF mediated EGFR transactivation",

abstract = "GPR48 can mediate keratinocyte proliferation and migration. Our investigations showed that AG1478, an inhibitor of EGFR tyrosine kinase, could block GPR48-mediated cellular processes. AG1478 treatment of Gpr48+/+ cells also decreased phosphorylation of EGFR, ERK and STAT3. Subsequent screening using conditioned media immunodepleted of EGFR ligands identified HB-EGF as the ligand responsible for phosphorylation of EGFR, ERK and STAT3. HB-EGF was reduced in Gpr48-/- cell culture medium, but its addition restored the phosphorylation of EGFR, ERK, STAT3, as well as cell proliferation. Confirmation that GPR48 mediates EGFR signaling pathway through HB-EGF was subsequently performed using an inhibitor of HB-EGF.",

keywords = "Cell proliferation, EGFR, GPR48, HB-EGF, Keratinocyte",

author = "Zhenlian Wang and Chang Jin and Hongxia Li and Canxia Li and Qiang Hou and Mingyao Liu and Dong, \{Xiang Da Eric\} and Tu, \{Li Li\}",

year = "2010",

month = sep,

doi = "10.1016/j.febslet.2010.08.028",

language = "英语",

volume = "584",

pages = "4057--4062",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc",

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}

TY - JOUR

T1 - GPR48-Induced keratinocyte proliferation occurs through HB-EGF mediated EGFR transactivation

AU - Wang, Zhenlian

AU - Jin, Chang

AU - Li, Hongxia

AU - Li, Canxia

AU - Hou, Qiang

AU - Liu, Mingyao

AU - Dong, Xiang Da Eric

AU - Tu, Li Li

PY - 2010/9

Y1 - 2010/9

N2 - GPR48 can mediate keratinocyte proliferation and migration. Our investigations showed that AG1478, an inhibitor of EGFR tyrosine kinase, could block GPR48-mediated cellular processes. AG1478 treatment of Gpr48+/+ cells also decreased phosphorylation of EGFR, ERK and STAT3. Subsequent screening using conditioned media immunodepleted of EGFR ligands identified HB-EGF as the ligand responsible for phosphorylation of EGFR, ERK and STAT3. HB-EGF was reduced in Gpr48-/- cell culture medium, but its addition restored the phosphorylation of EGFR, ERK, STAT3, as well as cell proliferation. Confirmation that GPR48 mediates EGFR signaling pathway through HB-EGF was subsequently performed using an inhibitor of HB-EGF.

AB - GPR48 can mediate keratinocyte proliferation and migration. Our investigations showed that AG1478, an inhibitor of EGFR tyrosine kinase, could block GPR48-mediated cellular processes. AG1478 treatment of Gpr48+/+ cells also decreased phosphorylation of EGFR, ERK and STAT3. Subsequent screening using conditioned media immunodepleted of EGFR ligands identified HB-EGF as the ligand responsible for phosphorylation of EGFR, ERK and STAT3. HB-EGF was reduced in Gpr48-/- cell culture medium, but its addition restored the phosphorylation of EGFR, ERK, STAT3, as well as cell proliferation. Confirmation that GPR48 mediates EGFR signaling pathway through HB-EGF was subsequently performed using an inhibitor of HB-EGF.

KW - Cell proliferation

KW - EGFR

KW - GPR48

KW - HB-EGF

KW - Keratinocyte

UR - https://www.scopus.com/pages/publications/77956929919

U2 - 10.1016/j.febslet.2010.08.028

DO - 10.1016/j.febslet.2010.08.028

M3 - 文章

C2 - 20732323

AN - SCOPUS:77956929919

SN - 0014-5793

VL - 584

SP - 4057

EP - 4062

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

GPR48-Induced keratinocyte proliferation occurs through HB-EGF mediated EGFR transactivation

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Keywords

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