@inbook{950b00212b9b4fc4b3b4510266aaaace,
title = "Generation of site-specific mutations in the rat genome via CRISPR/Cas9",
abstract = "The laboratory rat is a valuable model organism for basic biological studies and drug development. However, due to the lack of genetic tools for site-specific genetic modification in the rat genome, more and more researchers chose the mouse as their favored mammalian models due to the sophisticated embryonic stem cell-based gene-targeting techniques available. Recently, engineered nucleases, including zinc finger nucleases, transcription activator-like effector nucleases, and CRISPR/Cas9 systems, have been adapted to generate knockout rats efficiently. The purpose of this section is to provide detailed procedures for the generation of site-specific mutations in the rat genome through injection of Cas9/sgRNA into one-cell embryos.",
keywords = "Gene editing, Knockout Knockin, Mutation, One-cell embryo, Rat genome",
author = "Yuting Guan and Yanjiao Shao and Dali Li and Mingyao Liu",
note = "Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",
year = "2014",
doi = "10.1016/B978-0-12-801185-0.00014-3",
language = "英语",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
number = "C",
pages = "297--317",
booktitle = "Methods in Enzymology",
address = "美国",
edition = "C",
}