TY - JOUR
T1 - Fluorescence kinetics of Trp-Trp dipeptide and its derivatives in water via ultrafast fluorescence spectroscopy
AU - Jia, Menghui
AU - Yi, Hua
AU - Chang, Mengfang
AU - Cao, Xiaodan
AU - Li, Lei
AU - Zhou, Zhongneng
AU - Pan, Haifeng
AU - Chen, Yan
AU - Zhang, Sanjun
AU - Xu, Jianhua
N1 - Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/6/23
Y1 - 2015/6/23
N2 - Ultrafast fluorescence dynamics of Tryptophan-Tryptophan (Trp-Trp/Trp2) dipeptide and its derivatives in water have been investigated using a picosecond resolved time correlated single photon counting (TCSPC) apparatus together with a femtosecond resolved upconversion spectrophotofluorometer. The fluorescence decay profiles at multiple wavelengths were fitted by a global analysis technique. Nanosecond fluorescence kinetics of Trp2, N-tert-butyl carbonyl oxygen-N′-aldehyde group-l-tryptophan-l-tryptophan (NBTrp2), l-tryptophan-l-tryptophan methyl ester (Trp2Me), and N-acetyl-l-tryptophan-l-tryptophan methyl ester (NATrp2Me) exhibit multi-exponential decays with the average lifetimes of 1.99, 3.04, 0.72 and 1.22 ns, respectively. Due to the intramolecular interaction between two Trp residues, the "water relaxation" lifetime was observed around 4 ps, and it is noticed that Trp2 and its derivatives also exhibit a new decay with a lifetime of ∼100 ps, while single-Trp fluorescence decay in dipeptides/proteins shows 20-30 ps. The intramolecular interaction lifetime constants of Trp2, NBTrp2, Trp2Me and NATrp2Me were then calculated to be 3.64, 0.93, 11.52 and 2.40 ns, respectively. Candidate mechanisms (including heterogeneity, solvent relaxation, quasi static self-quenching or ET/PT quenching) have been discussed.
AB - Ultrafast fluorescence dynamics of Tryptophan-Tryptophan (Trp-Trp/Trp2) dipeptide and its derivatives in water have been investigated using a picosecond resolved time correlated single photon counting (TCSPC) apparatus together with a femtosecond resolved upconversion spectrophotofluorometer. The fluorescence decay profiles at multiple wavelengths were fitted by a global analysis technique. Nanosecond fluorescence kinetics of Trp2, N-tert-butyl carbonyl oxygen-N′-aldehyde group-l-tryptophan-l-tryptophan (NBTrp2), l-tryptophan-l-tryptophan methyl ester (Trp2Me), and N-acetyl-l-tryptophan-l-tryptophan methyl ester (NATrp2Me) exhibit multi-exponential decays with the average lifetimes of 1.99, 3.04, 0.72 and 1.22 ns, respectively. Due to the intramolecular interaction between two Trp residues, the "water relaxation" lifetime was observed around 4 ps, and it is noticed that Trp2 and its derivatives also exhibit a new decay with a lifetime of ∼100 ps, while single-Trp fluorescence decay in dipeptides/proteins shows 20-30 ps. The intramolecular interaction lifetime constants of Trp2, NBTrp2, Trp2Me and NATrp2Me were then calculated to be 3.64, 0.93, 11.52 and 2.40 ns, respectively. Candidate mechanisms (including heterogeneity, solvent relaxation, quasi static self-quenching or ET/PT quenching) have been discussed.
KW - Decay associated spectra (DAS)
KW - Electron/proton transfer (ET/PT)
KW - Quasi static self-quenching (QSSQ)
KW - Time resolved fluorescence
KW - Tryptophan dipeptides
UR - https://www.scopus.com/pages/publications/84934912864
U2 - 10.1016/j.jphotobiol.2015.06.014
DO - 10.1016/j.jphotobiol.2015.06.014
M3 - 文章
C2 - 26111991
AN - SCOPUS:84934912864
SN - 1011-1344
VL - 149
SP - 243
EP - 248
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
ER -