Filamentous green algae Spirogyra regulates methane emissions from eutrophic rivers

Excessive growth of filamentous green algae in rivers has attracted much attention due to their functional importance to primary production and carbon cycling. However, comprehensive knowledge of how filamentous green algae affect carbon cycling, especially the CH₄ emissions from river ecosystems, remains limited. In this study, incubation experiments were conducted to examine the factors regulating CH₄ emissions from a eutrophic river with dense growth of filamentous green algae Spirogyra through combinations of biogeochemical, molecular biological, and stable carbon isotope analyses. Results showed that although water dissolved oxygen (DO) in the algae+sediment (A+S) incubation groups increased up to 19 mg L⁻¹, average CH₄ flux of the groups was 13.09 μmol m⁻² day⁻¹, nearly up to two times higher than that from sediments without algae (S groups). The significant increase of sediment CH₄ oxidation potential and methanotroph abundances identified the enhancing sediment CH₄ oxidation during Spirogyra bloom. However, the increased water CH₄ concentration was consistent with depleted water δ13CCH4 and decreased apparent fractionation factor (α_app), suggesting the important contribution of Spirogyra to the oxic water CH₄ production. It can thus be concluded that high DO concentration during the algal bloom promoted the CH₄ consumption by enhancing sediment CH₄ oxidation, while algal-linked oxic water CH₄ production as a major component of water CH₄ promoted the CH₄ emissions from the river. Our study highlights the regulation of Spirogyra in aquatic CH₄ fluxes and will help to estimate accurately CH₄ emissions from eutrophic rivers with dense blooms of filamentous green algae. [Figure not available: see fulltext.]