Fifteen polymorphic microsatellite loci in wiebesia pumilae (Hill) (Agaonidae)

Min Liu; Xiao Yan Wang; Min Yan Cui; Yan Chen

doi:10.1007/s12686-009-9046-3

Fifteen polymorphic microsatellite loci in wiebesia pumilae (Hill) (Agaonidae)

Min Liu
, Xiao Yan Wang
, Min Yan Cui
, Yan Chen^*

^*Corresponding author for this work

School of Ecology and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae, the obligate pollinator of Ficus pumila, and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed (H _O) and expected (H _E) heterozygosities were 0.190-0.727 and 0.483-0.831, respectively. Significant deviation from Hardy-Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae, and its coevolution with the host F. pumila.

Original language	English
Pages (from-to)	189-191
Number of pages	3
Journal	Conservation Genetics Resources
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/s12686-009-9046-3
State	Published - Dec 2009

Keywords

Genetic diversity
Microsatellite loci
Mutualism
Wiebesia pumilae

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abstract = "Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae, the obligate pollinator of Ficus pumila, and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed (H O) and expected (H E) heterozygosities were 0.190-0.727 and 0.483-0.831, respectively. Significant deviation from Hardy-Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae, and its coevolution with the host F. pumila.",

keywords = "Genetic diversity, Microsatellite loci, Mutualism, Wiebesia pumilae",

author = "Min Liu and Wang, \{Xiao Yan\} and Cui, \{Min Yan\} and Yan Chen",

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doi = "10.1007/s12686-009-9046-3",

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T1 - Fifteen polymorphic microsatellite loci in wiebesia pumilae (Hill) (Agaonidae)

AU - Liu, Min

AU - Wang, Xiao Yan

AU - Cui, Min Yan

AU - Chen, Yan

PY - 2009/12

Y1 - 2009/12

N2 - Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae, the obligate pollinator of Ficus pumila, and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed (H O) and expected (H E) heterozygosities were 0.190-0.727 and 0.483-0.831, respectively. Significant deviation from Hardy-Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae, and its coevolution with the host F. pumila.

AB - Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae, the obligate pollinator of Ficus pumila, and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed (H O) and expected (H E) heterozygosities were 0.190-0.727 and 0.483-0.831, respectively. Significant deviation from Hardy-Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae, and its coevolution with the host F. pumila.

KW - Genetic diversity

KW - Microsatellite loci

KW - Mutualism

KW - Wiebesia pumilae

UR - https://www.scopus.com/pages/publications/84859958048

U2 - 10.1007/s12686-009-9046-3

DO - 10.1007/s12686-009-9046-3

M3 - 文章

AN - SCOPUS:84859958048

SN - 1877-7252

VL - 1

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EP - 191

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

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ER -

Fifteen polymorphic microsatellite loci in wiebesia pumilae (Hill) (Agaonidae)

Abstract

Keywords

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