Fermentation liquor of CaO₂ treated chemically enhanced primary sedimentation (CEPS) sludge for bioplastic biosynthesis

Chemically enhanced primary sedimentation (CEPS) technology has been widely applied in Hong Kong, exhibiting excellent performance in contaminants removal from sewage. The generated CEPS sludge contains abundance of organics which could be recovered as volatile fatty acids (VFAs) by fermentation for further utilization. In this work, the effect of calcium peroxide (CaO₂) on the fermentation of FeCl₃ based CEPS sludge was investigated. The feasibility of utilizing the fermentation liquor as substrate for polyhydroxyalkanoates (PHAs) biosynthesis was also evaluated. Results demonstrated that CaO₂ addition facilitated the disintegration of CEPS sludge and enhanced VFAs production. The maximum VFAs yield of 455.8 mg COD/g VSS was obtained with the dosage of 0.1 g CaO₂/g SS, improving by 44.7% compared with the control sludge. Acetic and propionic acid were the predominant components of the VFAs. Microbial analysis indicated that CaO₂ induced microbial reduction of Fe(III), accelerating the initial disintegration of FeCl₃ based CEPS sludge. Microbial communities with hydrolysis and acidogenesis functions were enriched effectively. CaO₂ treatment had no significant influence on the release of ammonia nitrogen (NH₄ ⁺-N), while reduced the concentration of orthophosphate (PO₄ ³⁻-P) and ferrous (Fe²⁺) in fermentation liquor, that was beneficial to the further utilization as substrate for PHAs biosynthesis. The VFA-rich fermentation liquor was proved to be a suitable substrate for PHAs biosynthesis. After cultivation, the PHAs content in activated sludge reached 22.3%, which was comparable to those obtained using waste materials as carbon source. This integrated technology could be a superior alternative of realizing sludge disposal and bioplastic production simultaneously.