FAN: Fourier Assignment Network for autofocus based on deep learning

Qizheng Liu, Xintian Mao, Jiansheng Wang, Qing Zhang, Yan Wang, Baochuan Pang, Qingli Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

AI-driven digital pathology is transforming the field, prioritizing the enhancement of image quality. In the data acquisition phase, the performance of autofocus algorithms significantly influences imaging quality. To tackle this challenge, we introduce the Fourier Assignment Network (FAN), a novel and lightweight neural network architecture aimed at predicting the defocus distance in microscopic images. FAN estimates the defocus distance using single-shot images, thus eliminating the requirement for extra hardware. Extensive experiments conducted on our dataset confirm its effectiveness, with an average error of 0.9μm across 1374 sample groups, markedly surpassing the performance of existing lightweight neural networks. Moreover, FAN is the most efficient among surveyed networks, with only 0.14G FLOPs and 0.85M parameters. The real-time and precise autofocus capability of FAN marks a notable advancement in digital pathology, providing a dependable, hardware-independent solution to enhance diagnostic accuracy.

Original languageEnglish
Article number112579
JournalOptics and Laser Technology
Volume186
DOIs
StatePublished - Aug 2025

Keywords

  • Autofocus
  • Deep learning
  • Digital pathology
  • Microscopy

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