Fabrication of near infrared light responsive photoelectrochemical immunosensor for in vivo detection of melanoma cells

Bing Wang; Chuan Yuan Wei; Kang Wei Wang; Baihe Fu; Yong Chen; Yu Han; Zhonghai Zhang

doi:10.1016/j.bios.2023.115601

Fabrication of near infrared light responsive photoelectrochemical immunosensor for in vivo detection of melanoma cells

Bing Wang
, Chuan Yuan Wei
, Kang Wei Wang
, Baihe Fu
, Yong Chen^*
, Yu Han^*
, Zhonghai Zhang^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Effective and convenient detection of melanoma cells with high sensitivity is essential to identify malignant melanoma in its early stage. However, the existing detection methods, such as immunohistochemical analysis, are too complicated and time-consuming to realize the convenient in vivo and in situ detection. Herein, a near infrared responsive photoelectrochemical (PEC) immunosensor is proposed with plasmonic Au nanoparticles-photonic TiO₂ nanocaves (Au/TiO₂ NCs) as photon harvest and conversion transducer and antibody as cell recognition unit. The micro-antibody/Au/TiO₂ NCs photoelectrode can easily in vivo distinguish melanoma cells and can realize sensitive detection of melanoma cells in short time of 1 min with a lowest limit of detection of 2 cell mL⁻¹. The PEC immunosensor strategy not only allows us to pioneeringly implement sensitive in vivo bio-detection, but also opens up a new avenue for rational design of cell recognition units and micro-electrode for universal and reliable bio-detections.

Original language	English
Article number	115601
Journal	Biosensors and Bioelectronics
Volume	239
DOIs	https://doi.org/10.1016/j.bios.2023.115601
State	Published - 1 Nov 2023

Keywords

Au nanoparticles
Immunosensor
Melanoma cells
Photoelectrochemical analysis
TiO nanocave

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10.1016/j.bios.2023.115601

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@article{a4c0395234a94e9c8097e830e7090db9,

title = "Fabrication of near infrared light responsive photoelectrochemical immunosensor for in vivo detection of melanoma cells",

abstract = "Effective and convenient detection of melanoma cells with high sensitivity is essential to identify malignant melanoma in its early stage. However, the existing detection methods, such as immunohistochemical analysis, are too complicated and time-consuming to realize the convenient in vivo and in situ detection. Herein, a near infrared responsive photoelectrochemical (PEC) immunosensor is proposed with plasmonic Au nanoparticles-photonic TiO2 nanocaves (Au/TiO2 NCs) as photon harvest and conversion transducer and antibody as cell recognition unit. The micro-antibody/Au/TiO2 NCs photoelectrode can easily in vivo distinguish melanoma cells and can realize sensitive detection of melanoma cells in short time of 1 min with a lowest limit of detection of 2 cell mL−1. The PEC immunosensor strategy not only allows us to pioneeringly implement sensitive in vivo bio-detection, but also opens up a new avenue for rational design of cell recognition units and micro-electrode for universal and reliable bio-detections.",

keywords = "Au nanoparticles, Immunosensor, Melanoma cells, Photoelectrochemical analysis, TiO nanocave",

author = "Bing Wang and Wei, \{Chuan Yuan\} and Wang, \{Kang Wei\} and Baihe Fu and Yong Chen and Yu Han and Zhonghai Zhang",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2023",

month = nov,

day = "1",

doi = "10.1016/j.bios.2023.115601",

language = "英语",

volume = "239",

journal = "Biosensors and Bioelectronics",

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}

TY - JOUR

T1 - Fabrication of near infrared light responsive photoelectrochemical immunosensor for in vivo detection of melanoma cells

AU - Wang, Bing

AU - Wei, Chuan Yuan

AU - Wang, Kang Wei

AU - Fu, Baihe

AU - Chen, Yong

AU - Han, Yu

AU - Zhang, Zhonghai

PY - 2023/11/1

Y1 - 2023/11/1

N2 - Effective and convenient detection of melanoma cells with high sensitivity is essential to identify malignant melanoma in its early stage. However, the existing detection methods, such as immunohistochemical analysis, are too complicated and time-consuming to realize the convenient in vivo and in situ detection. Herein, a near infrared responsive photoelectrochemical (PEC) immunosensor is proposed with plasmonic Au nanoparticles-photonic TiO2 nanocaves (Au/TiO2 NCs) as photon harvest and conversion transducer and antibody as cell recognition unit. The micro-antibody/Au/TiO2 NCs photoelectrode can easily in vivo distinguish melanoma cells and can realize sensitive detection of melanoma cells in short time of 1 min with a lowest limit of detection of 2 cell mL−1. The PEC immunosensor strategy not only allows us to pioneeringly implement sensitive in vivo bio-detection, but also opens up a new avenue for rational design of cell recognition units and micro-electrode for universal and reliable bio-detections.

AB - Effective and convenient detection of melanoma cells with high sensitivity is essential to identify malignant melanoma in its early stage. However, the existing detection methods, such as immunohistochemical analysis, are too complicated and time-consuming to realize the convenient in vivo and in situ detection. Herein, a near infrared responsive photoelectrochemical (PEC) immunosensor is proposed with plasmonic Au nanoparticles-photonic TiO2 nanocaves (Au/TiO2 NCs) as photon harvest and conversion transducer and antibody as cell recognition unit. The micro-antibody/Au/TiO2 NCs photoelectrode can easily in vivo distinguish melanoma cells and can realize sensitive detection of melanoma cells in short time of 1 min with a lowest limit of detection of 2 cell mL−1. The PEC immunosensor strategy not only allows us to pioneeringly implement sensitive in vivo bio-detection, but also opens up a new avenue for rational design of cell recognition units and micro-electrode for universal and reliable bio-detections.

KW - Au nanoparticles

KW - Immunosensor

KW - Melanoma cells

KW - Photoelectrochemical analysis

KW - TiO nanocave

UR - https://www.scopus.com/pages/publications/85169846752

U2 - 10.1016/j.bios.2023.115601

DO - 10.1016/j.bios.2023.115601

M3 - 文章

C2 - 37633000

AN - SCOPUS:85169846752

SN - 0956-5663

VL - 239

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

M1 - 115601

ER -

Fabrication of near infrared light responsive photoelectrochemical immunosensor for in vivo detection of melanoma cells

Abstract

Keywords

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