TY - JOUR
T1 - Fabrication of Calcium Phosphate-Based Nanocomposites Incorporating DNA Origami, Gold Nanorods, and Anticancer Drugs for Biomedical Applications
AU - Zhang, Hongbo
AU - Qu, Xiangmeng
AU - Chen, Hong
AU - Kong, Haixin
AU - Ding, Ruihua
AU - Chen, Dong
AU - Zhang, Xu
AU - Pei, Hao
AU - Santos, Hélder A.
AU - Hai, Mingtan
AU - Weitz, David A.
N1 - Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/10/25
Y1 - 2017/10/25
N2 - DNA origami is designed by folding DNA strands at the nanoscale with arbitrary control. Due to its inherent biological nature, DNA origami is used in drug delivery for enhancement of synergism and multidrug resistance inhibition, cancer diagnosis, and many other biomedical applications, where it shows great potential. However, the inherent instability and low payload capacity of DNA origami restrict its biomedical applications. Here, this paper reports the fabrication of an advanced biocompatible nano-in-nanocomposite, which protects DNA origami from degradation and facilities drug loading. The DNA origami, gold nanorods, and molecular targeted drugs are co-incorporated into pH responsive calcium phosphate [Ca3(PO4)2] nanoparticles. Subsequently, a thin layer of phospholipid is coated onto the Ca3(PO4)2 nanoparticle to offer better biocompatibility. The fabricated nanocomposite shows high drug loading capacity, good biocompatibility, and a photothermal and pH-responsive payload release profile and it fully protects DNA origami from degradation. The codelivery of DNA origami with cancer drugs synergistically induces cancer cell apoptosis, reduces the multidrug resistance, and enhances the targeted killing efficiency toward human epidermal growth factor receptor 2 positive cells. This nanocomposite is foreseen to open new horizons for a variety of clinical and biomedical applications.
AB - DNA origami is designed by folding DNA strands at the nanoscale with arbitrary control. Due to its inherent biological nature, DNA origami is used in drug delivery for enhancement of synergism and multidrug resistance inhibition, cancer diagnosis, and many other biomedical applications, where it shows great potential. However, the inherent instability and low payload capacity of DNA origami restrict its biomedical applications. Here, this paper reports the fabrication of an advanced biocompatible nano-in-nanocomposite, which protects DNA origami from degradation and facilities drug loading. The DNA origami, gold nanorods, and molecular targeted drugs are co-incorporated into pH responsive calcium phosphate [Ca3(PO4)2] nanoparticles. Subsequently, a thin layer of phospholipid is coated onto the Ca3(PO4)2 nanoparticle to offer better biocompatibility. The fabricated nanocomposite shows high drug loading capacity, good biocompatibility, and a photothermal and pH-responsive payload release profile and it fully protects DNA origami from degradation. The codelivery of DNA origami with cancer drugs synergistically induces cancer cell apoptosis, reduces the multidrug resistance, and enhances the targeted killing efficiency toward human epidermal growth factor receptor 2 positive cells. This nanocomposite is foreseen to open new horizons for a variety of clinical and biomedical applications.
KW - DNA origami
KW - biomedical applications
KW - calcium phosphate
KW - cancer treatment
KW - gold nanorods
UR - https://www.scopus.com/pages/publications/85030229661
U2 - 10.1002/adhm.201700664
DO - 10.1002/adhm.201700664
M3 - 文章
C2 - 28941223
AN - SCOPUS:85030229661
SN - 2192-2640
VL - 6
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 20
M1 - 1700664
ER -
