Fabrication of a micro-optical lens using femtosecond laser 3D micromachining for two-photon imaging of bio-tissues

Lingling Qiao; Fei He; Chen Wang; Yang Liao; Ya Cheng; Koji Sugioka; Katsumi Midorikawa; Change Pan; Xunbin Wei

doi:10.1016/j.optcom.2011.01.082

Fabrication of a micro-optical lens using femtosecond laser 3D micromachining for two-photon imaging of bio-tissues

Lingling Qiao
, Fei He
, Chen Wang
, Yang Liao
, Ya Cheng^*
, Koji Sugioka
, Katsumi Midorikawa
, Change Pan
, Xunbin Wei

^*Corresponding author for this work

CAS - Shanghai Institute of Optics and Fine Mechanics
University of Chinese Academy of Sciences
RIKEN
Fudan University

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We report, for the first time to our knowledge, on the application of a micro-optical lens fabricated by three-dimensional (3D) femtosecond laser direct writing for two-photon fluorescence imaging of biological tissues. We show that the two-photon fluorescence images of a plant leaf tissue acquired with the micro-optical lens are comparable to that of a 5× objective lens. Our result represents an important step towards the application of micro-optical components fabricated by femtosecond laser micromachining in miniaturized nonlinear fluorescence microscopy applications, such as two-photon endoscopy.

Original language	English
Pages (from-to)	2988-2991
Number of pages	4
Journal	Optics Communications
Volume	284
Issue number	12
DOIs	https://doi.org/10.1016/j.optcom.2011.01.082
State	Published - 1 Jun 2011
Externally published	Yes

Keywords

Bio-tissue
Femtosecond laser micromachining
Micro-optical lens
Surface roughness
Two-photon imaging

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10.1016/j.optcom.2011.01.082

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title = "Fabrication of a micro-optical lens using femtosecond laser 3D micromachining for two-photon imaging of bio-tissues",

abstract = "We report, for the first time to our knowledge, on the application of a micro-optical lens fabricated by three-dimensional (3D) femtosecond laser direct writing for two-photon fluorescence imaging of biological tissues. We show that the two-photon fluorescence images of a plant leaf tissue acquired with the micro-optical lens are comparable to that of a 5× objective lens. Our result represents an important step towards the application of micro-optical components fabricated by femtosecond laser micromachining in miniaturized nonlinear fluorescence microscopy applications, such as two-photon endoscopy.",

keywords = "Bio-tissue, Femtosecond laser micromachining, Micro-optical lens, Surface roughness, Two-photon imaging",

author = "Lingling Qiao and Fei He and Chen Wang and Yang Liao and Ya Cheng and Koji Sugioka and Katsumi Midorikawa and Change Pan and Xunbin Wei",

year = "2011",

month = jun,

day = "1",

doi = "10.1016/j.optcom.2011.01.082",

language = "英语",

volume = "284",

pages = "2988--2991",

journal = "Optics Communications",

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T1 - Fabrication of a micro-optical lens using femtosecond laser 3D micromachining for two-photon imaging of bio-tissues

AU - Qiao, Lingling

AU - He, Fei

AU - Wang, Chen

AU - Liao, Yang

AU - Cheng, Ya

AU - Sugioka, Koji

AU - Midorikawa, Katsumi

AU - Pan, Change

AU - Wei, Xunbin

PY - 2011/6/1

Y1 - 2011/6/1

N2 - We report, for the first time to our knowledge, on the application of a micro-optical lens fabricated by three-dimensional (3D) femtosecond laser direct writing for two-photon fluorescence imaging of biological tissues. We show that the two-photon fluorescence images of a plant leaf tissue acquired with the micro-optical lens are comparable to that of a 5× objective lens. Our result represents an important step towards the application of micro-optical components fabricated by femtosecond laser micromachining in miniaturized nonlinear fluorescence microscopy applications, such as two-photon endoscopy.

AB - We report, for the first time to our knowledge, on the application of a micro-optical lens fabricated by three-dimensional (3D) femtosecond laser direct writing for two-photon fluorescence imaging of biological tissues. We show that the two-photon fluorescence images of a plant leaf tissue acquired with the micro-optical lens are comparable to that of a 5× objective lens. Our result represents an important step towards the application of micro-optical components fabricated by femtosecond laser micromachining in miniaturized nonlinear fluorescence microscopy applications, such as two-photon endoscopy.

KW - Bio-tissue

KW - Femtosecond laser micromachining

KW - Micro-optical lens

KW - Surface roughness

KW - Two-photon imaging

UR - https://www.scopus.com/pages/publications/79954994381

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DO - 10.1016/j.optcom.2011.01.082

M3 - 文章

AN - SCOPUS:79954994381

SN - 0030-4018

VL - 284

SP - 2988

EP - 2991

JO - Optics Communications

JF - Optics Communications

IS - 12

ER -

Fabrication of a micro-optical lens using femtosecond laser 3D micromachining for two-photon imaging of bio-tissues

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Keywords

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