Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex

Jie Zhou; Yanyan Jia; Xueli Wang; Menghui Jia; Haifeng Pan; Zhenrong Sun; Jinquan Chen

doi:10.3390/molecules27238157

Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex

Jie Zhou, Yanyan Jia^*, Xueli Wang, Menghui Jia, Haifeng Pan, Zhenrong Sun, Jinquan Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed.

Original language	English
Article number	8157
Journal	Molecules
Volume	27
Issue number	23
DOIs	https://doi.org/10.3390/molecules27238157
State	Published - Dec 2022
Externally published	Yes

Keywords

DNA duplex
excited-state dynamics
femtosecond transient-absorption
fluorescence-quenching
proflavine

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10.3390/molecules27238157

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@article{99e9f9abaf094bb0bd1f9cc00c9f9e14,

title = "Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex",

abstract = "Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed.",

keywords = "DNA duplex, excited-state dynamics, femtosecond transient-absorption, fluorescence-quenching, proflavine",

author = "Jie Zhou and Yanyan Jia and Xueli Wang and Menghui Jia and Haifeng Pan and Zhenrong Sun and Jinquan Chen",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = dec,

doi = "10.3390/molecules27238157",

language = "英语",

volume = "27",

journal = "Molecules",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "23",

}

TY - JOUR

T1 - Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex

AU - Zhou, Jie

AU - Jia, Yanyan

AU - Wang, Xueli

AU - Jia, Menghui

AU - Pan, Haifeng

AU - Sun, Zhenrong

AU - Chen, Jinquan

PY - 2022/12

Y1 - 2022/12

N2 - Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed.

AB - Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed.

KW - DNA duplex

KW - excited-state dynamics

KW - femtosecond transient-absorption

KW - fluorescence-quenching

KW - proflavine

UR - https://www.scopus.com/pages/publications/85143625051

U2 - 10.3390/molecules27238157

DO - 10.3390/molecules27238157

M3 - 文章

C2 - 36500248

AN - SCOPUS:85143625051

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 23

M1 - 8157

ER -

Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex

Abstract

Keywords

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