TY - JOUR
T1 - Evalution of the synergistic effects of cholesterol and phospholipid on ovarian lipid accumulation, steroidogenesis and vitellogenesis in female broodstock in the Pacific whiteleg shrimp, Litopenaeus vannamei
AU - Liang, Xiaolong
AU - Zhang, Long
AU - Chang, Tong
AU - Wang, Biao
AU - Zhang, Cunlai
AU - Han, Fenglu
AU - Xu, Chang
AU - Li, Erchao
N1 - Publisher Copyright:
© 2024
PY - 2026/2/1
Y1 - 2026/2/1
N2 - Lipid nutrition is a critical regulator of ovarian development and maturation in the Pacific whiteleg shrimp Litopenaeus vannamei broodstock. Previous studies have demonstrated a close association between cholesterol (CHO) and phospholipid (PL) metabolism during the reproductive process in shrimp. However, the specific interactive effects of CHO and PL on the regulation of reproduction in L. vannamei remain unclear. This study systematically analyzed the effects of different CHO and PL ratios on ovarian development and elucidated the synergistic mechanisms by which these lipids promote ovarian maturation through multiple metabolic pathways. The results demonstrated that dietary CHO and PL had significant interactive effects on the gonadosomatic index (GSI), hepatosomatic index (HSI), ovarian triglyceride (TG), hepatopancreatic total cholesterol (T-CHO) content, serum low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) levels, vitellogenin (LvVg1) expression in the hepatopancreas and ovary, and vitellogenin receptor (Lv-VgR) expression in the ovary. Specifically, the C1P4 group presented the best performance, characterized by higher GSI, HSI, hepatopancreatic and ovarian TG/T-CHO content, serum LDL/VLDL levels, and estradiol (E2) and progesterone (P4) concentrations than the control and other treatment groups. CHO and PL supplementation significantly suppressed gonad-inhibiting hormone (GIH) levels. Additionally, the combined supplementation with CHO and PL significantly enhanced the stimulatory effect of CHO on the synthesis of sex steroid hormones (E2 and P4), whereas PL primarily influenced lipid accumulation (TG/T-CHO) in the hepatopancreas and ovaries. At the metabolic level, the synergistic effects of CHO and PL further enhanced the regulation of amino acids (L-valine and L-aspartic acid) and lipids (linoleic acid and γ-linolenic acid), providing essential substrates and energy for ovarian development. Furthermore, this combination modulated the arachidonic acid metabolic pathway, promoting the production of its metabolites (prostaglandin H2 and 8-HETE), thereby improving the efficiency of sex steroid hormone synthesis. In conclusion, an optimal CHO:PL ratio (1 % CHO + 4 % PL) facilitated ovarian maturation by modulating lipid accumulation, hormone secretion, and vitellogenin synthesis networks. These findings provide new insights into the optimization of nutritional strategies for L. vannamei.
AB - Lipid nutrition is a critical regulator of ovarian development and maturation in the Pacific whiteleg shrimp Litopenaeus vannamei broodstock. Previous studies have demonstrated a close association between cholesterol (CHO) and phospholipid (PL) metabolism during the reproductive process in shrimp. However, the specific interactive effects of CHO and PL on the regulation of reproduction in L. vannamei remain unclear. This study systematically analyzed the effects of different CHO and PL ratios on ovarian development and elucidated the synergistic mechanisms by which these lipids promote ovarian maturation through multiple metabolic pathways. The results demonstrated that dietary CHO and PL had significant interactive effects on the gonadosomatic index (GSI), hepatosomatic index (HSI), ovarian triglyceride (TG), hepatopancreatic total cholesterol (T-CHO) content, serum low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) levels, vitellogenin (LvVg1) expression in the hepatopancreas and ovary, and vitellogenin receptor (Lv-VgR) expression in the ovary. Specifically, the C1P4 group presented the best performance, characterized by higher GSI, HSI, hepatopancreatic and ovarian TG/T-CHO content, serum LDL/VLDL levels, and estradiol (E2) and progesterone (P4) concentrations than the control and other treatment groups. CHO and PL supplementation significantly suppressed gonad-inhibiting hormone (GIH) levels. Additionally, the combined supplementation with CHO and PL significantly enhanced the stimulatory effect of CHO on the synthesis of sex steroid hormones (E2 and P4), whereas PL primarily influenced lipid accumulation (TG/T-CHO) in the hepatopancreas and ovaries. At the metabolic level, the synergistic effects of CHO and PL further enhanced the regulation of amino acids (L-valine and L-aspartic acid) and lipids (linoleic acid and γ-linolenic acid), providing essential substrates and energy for ovarian development. Furthermore, this combination modulated the arachidonic acid metabolic pathway, promoting the production of its metabolites (prostaglandin H2 and 8-HETE), thereby improving the efficiency of sex steroid hormone synthesis. In conclusion, an optimal CHO:PL ratio (1 % CHO + 4 % PL) facilitated ovarian maturation by modulating lipid accumulation, hormone secretion, and vitellogenin synthesis networks. These findings provide new insights into the optimization of nutritional strategies for L. vannamei.
KW - Broodstock
KW - Cholesterol
KW - Interaction
KW - Litopenaeus vannamei
KW - Ovarian development
KW - Phospholipid
UR - https://www.scopus.com/pages/publications/105019780720
U2 - 10.1016/j.aquaculture.2025.743351
DO - 10.1016/j.aquaculture.2025.743351
M3 - 文章
AN - SCOPUS:105019780720
SN - 0044-8486
VL - 613
JO - Aquaculture
JF - Aquaculture
M1 - 743351
ER -