TY - JOUR
T1 - Enhancement of nitrite production via addition of hydroxylamine to partial denitrification (PD) biomass
T2 - Functional genes dynamics and enzymatic activities
AU - Zhang, Xingxing
AU - Xia, Yunkang
AU - Wang, Chaochao
AU - Li, Jiajia
AU - Wu, Peng
AU - Ma, Liping
AU - Wang, Yuguang
AU - Wang, Yao
AU - Da, Fanghua
AU - Liu, Wenru
AU - Xu, Lezhong
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/12
Y1 - 2020/12
N2 - This study investigated the activity of partial denitrification (PD) biomass/key enzymes, functional gene expressions in response to 0 ~ 50 mg/L hydroxylamine (NH2OH) addition. Results indicated that NH2OH contributed to nitrite (NO2−-N) production, facilitating the maximum increase of nitrate (NO3−-N) to NO2−-N transformation ratio to 80.47 ± 2.82%, leading to 2.56-fold NO2−-N higher than those of control. The observed transient inhibitory effect on NO3−-N reduction process was attributed by high-level NH2OH (35 ~ 50 mg/L). Enzymatic assays revealed the enhanced activity of both NO3−-N and NO2−-N reductase while the former showed obvious superiority which led to high NO2−-N accumulation. These results were further confirmed by the corresponding functional genes (narG, napA, nirS and nirK). Besides, negative influence of NH2OH addition was limited to PD aggregates, due to the increasing secretion of extracellular polymeric substances (EPS) as well as proteins/polysaccharides ratios in tightly-bound structure of EPS.
AB - This study investigated the activity of partial denitrification (PD) biomass/key enzymes, functional gene expressions in response to 0 ~ 50 mg/L hydroxylamine (NH2OH) addition. Results indicated that NH2OH contributed to nitrite (NO2−-N) production, facilitating the maximum increase of nitrate (NO3−-N) to NO2−-N transformation ratio to 80.47 ± 2.82%, leading to 2.56-fold NO2−-N higher than those of control. The observed transient inhibitory effect on NO3−-N reduction process was attributed by high-level NH2OH (35 ~ 50 mg/L). Enzymatic assays revealed the enhanced activity of both NO3−-N and NO2−-N reductase while the former showed obvious superiority which led to high NO2−-N accumulation. These results were further confirmed by the corresponding functional genes (narG, napA, nirS and nirK). Besides, negative influence of NH2OH addition was limited to PD aggregates, due to the increasing secretion of extracellular polymeric substances (EPS) as well as proteins/polysaccharides ratios in tightly-bound structure of EPS.
KW - Enzymatic activities
KW - Extracellular polymeric substances (EPS)
KW - Functional genes
KW - Hydroxylamine (NHOH) exposure
KW - Partial denitrification (PD)
UR - https://www.scopus.com/pages/publications/85092922451
U2 - 10.1016/j.biortech.2020.124274
DO - 10.1016/j.biortech.2020.124274
M3 - 文章
C2 - 33096441
AN - SCOPUS:85092922451
SN - 0960-8524
VL - 318
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 124274
ER -
