Encapsulation hemoglobin in ordered mesoporous silicas: Influence factors for immobilization and bioelectrochemistry

Yuezhong Xian; Yang Xian; Lihui Zhou; Fanghua Wu; Yun Ling; Litong Jin

doi:10.1016/j.elecom.2006.08.049

Encapsulation hemoglobin in ordered mesoporous silicas: Influence factors for immobilization and bioelectrochemistry

Yuezhong Xian
, Yang Xian
, Lihui Zhou
, Fanghua Wu
, Yun Ling
, Litong Jin^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

The encapsulation of hemoglobin (Hb) on the mesoporous silicas SBA-15 and Au-doped SBA-15 (Au-SBA-15) has been studied as a model protein adsorption system. The influences of solution pH, structure of mesoporous silicas and gold nanoparticles incorporation on Hb immobilization are investigated in detail. The spectral characteristics of Hb/SBA-15 and Hb/Au-SBA-15 nanoconjugate show an absorption curve quite similar to that of native Hb, indicating that Hb retains its higher-order structure in the mesopores of SBA-15. Direct electrochemistry of Hb is obtained when Hb is adsorpted by mesoporous silicas SBA-15 or Au-SBA-15. Moreover, Hb/Au-SBA-15 exerts enhancing electron transfer ability because of the Au incorporation. Additionally, the Hb/Au-SBA-15 displays good electrocatalytic reduction of hydrogen peroxide with a detection limit of 1.0 μM, about 3 times as low as that for the Hb/SBA-15. The Hb/Au-SBA-15 exhibits higher peroxidase-like activity with the apparent Michaelis-Menton constant (K_m) of 2.87 mM, significantly lower than the 7.78 mM value for the Hb/SBA-15.

Original language	English
Pages (from-to)	142-148
Number of pages	7
Journal	Electrochemistry Communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/j.elecom.2006.08.049
State	Published - Jan 2007

Keywords

Au-SBA-15
Direct chemistry
Hemoglobin
Hydrogen peroxide
SBA-15

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10.1016/j.elecom.2006.08.049

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@article{89cb4a5bbffd4feaa299710099796c42,

title = "Encapsulation hemoglobin in ordered mesoporous silicas: Influence factors for immobilization and bioelectrochemistry",

abstract = "The encapsulation of hemoglobin (Hb) on the mesoporous silicas SBA-15 and Au-doped SBA-15 (Au-SBA-15) has been studied as a model protein adsorption system. The influences of solution pH, structure of mesoporous silicas and gold nanoparticles incorporation on Hb immobilization are investigated in detail. The spectral characteristics of Hb/SBA-15 and Hb/Au-SBA-15 nanoconjugate show an absorption curve quite similar to that of native Hb, indicating that Hb retains its higher-order structure in the mesopores of SBA-15. Direct electrochemistry of Hb is obtained when Hb is adsorpted by mesoporous silicas SBA-15 or Au-SBA-15. Moreover, Hb/Au-SBA-15 exerts enhancing electron transfer ability because of the Au incorporation. Additionally, the Hb/Au-SBA-15 displays good electrocatalytic reduction of hydrogen peroxide with a detection limit of 1.0 μM, about 3 times as low as that for the Hb/SBA-15. The Hb/Au-SBA-15 exhibits higher peroxidase-like activity with the apparent Michaelis-Menton constant (Km) of 2.87 mM, significantly lower than the 7.78 mM value for the Hb/SBA-15.",

keywords = "Au-SBA-15, Direct chemistry, Hemoglobin, Hydrogen peroxide, SBA-15",

author = "Yuezhong Xian and Yang Xian and Lihui Zhou and Fanghua Wu and Yun Ling and Litong Jin",

year = "2007",

month = jan,

doi = "10.1016/j.elecom.2006.08.049",

language = "英语",

volume = "9",

pages = "142--148",

journal = "Electrochemistry Communications",

issn = "1388-2481",

publisher = "Elsevier Inc.",

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}

TY - JOUR

T1 - Encapsulation hemoglobin in ordered mesoporous silicas

T2 - Influence factors for immobilization and bioelectrochemistry

AU - Xian, Yuezhong

AU - Xian, Yang

AU - Zhou, Lihui

AU - Wu, Fanghua

AU - Ling, Yun

AU - Jin, Litong

PY - 2007/1

Y1 - 2007/1

N2 - The encapsulation of hemoglobin (Hb) on the mesoporous silicas SBA-15 and Au-doped SBA-15 (Au-SBA-15) has been studied as a model protein adsorption system. The influences of solution pH, structure of mesoporous silicas and gold nanoparticles incorporation on Hb immobilization are investigated in detail. The spectral characteristics of Hb/SBA-15 and Hb/Au-SBA-15 nanoconjugate show an absorption curve quite similar to that of native Hb, indicating that Hb retains its higher-order structure in the mesopores of SBA-15. Direct electrochemistry of Hb is obtained when Hb is adsorpted by mesoporous silicas SBA-15 or Au-SBA-15. Moreover, Hb/Au-SBA-15 exerts enhancing electron transfer ability because of the Au incorporation. Additionally, the Hb/Au-SBA-15 displays good electrocatalytic reduction of hydrogen peroxide with a detection limit of 1.0 μM, about 3 times as low as that for the Hb/SBA-15. The Hb/Au-SBA-15 exhibits higher peroxidase-like activity with the apparent Michaelis-Menton constant (Km) of 2.87 mM, significantly lower than the 7.78 mM value for the Hb/SBA-15.

AB - The encapsulation of hemoglobin (Hb) on the mesoporous silicas SBA-15 and Au-doped SBA-15 (Au-SBA-15) has been studied as a model protein adsorption system. The influences of solution pH, structure of mesoporous silicas and gold nanoparticles incorporation on Hb immobilization are investigated in detail. The spectral characteristics of Hb/SBA-15 and Hb/Au-SBA-15 nanoconjugate show an absorption curve quite similar to that of native Hb, indicating that Hb retains its higher-order structure in the mesopores of SBA-15. Direct electrochemistry of Hb is obtained when Hb is adsorpted by mesoporous silicas SBA-15 or Au-SBA-15. Moreover, Hb/Au-SBA-15 exerts enhancing electron transfer ability because of the Au incorporation. Additionally, the Hb/Au-SBA-15 displays good electrocatalytic reduction of hydrogen peroxide with a detection limit of 1.0 μM, about 3 times as low as that for the Hb/SBA-15. The Hb/Au-SBA-15 exhibits higher peroxidase-like activity with the apparent Michaelis-Menton constant (Km) of 2.87 mM, significantly lower than the 7.78 mM value for the Hb/SBA-15.

KW - Au-SBA-15

KW - Direct chemistry

KW - Hemoglobin

KW - Hydrogen peroxide

KW - SBA-15

UR - https://www.scopus.com/pages/publications/33845342565

U2 - 10.1016/j.elecom.2006.08.049

DO - 10.1016/j.elecom.2006.08.049

M3 - 文章

AN - SCOPUS:33845342565

SN - 1388-2481

VL - 9

SP - 142

EP - 148

JO - Electrochemistry Communications

JF - Electrochemistry Communications

IS - 1

ER -

Encapsulation hemoglobin in ordered mesoporous silicas: Influence factors for immobilization and bioelectrochemistry

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Keywords

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