Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

Ying Xu Zeng; Pedro Araujo; Zhen Yu Du; Thu Thao Nguyen; Livar Frøyland; Bjørn Grung

doi:10.1016/j.talanta.2010.06.055

Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

Ying Xu Zeng
, Pedro Araujo^*
, Zhen Yu Du
, Thu Thao Nguyen
, Livar Frøyland
, Bjørn Grung

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS²) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS ² for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.

Original language	English
Pages (from-to)	1261-1270
Number of pages	10
Journal	Talanta
Volume	82
Issue number	4
DOIs	https://doi.org/10.1016/j.talanta.2010.06.055
State	Published - 15 Sep 2010
Externally published	Yes

Keywords

Algorithm
Cod liver oil
Fatty acids
Liquid chromatography electrospray tandem mass spectrometry
Triacylglycerols

Access to Document

10.1016/j.talanta.2010.06.055

Cite this

@article{1db84721033244fbbeb4f87019e22ae0,

title = "Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry",

abstract = "Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS2) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS 2 for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.",

keywords = "Algorithm, Cod liver oil, Fatty acids, Liquid chromatography electrospray tandem mass spectrometry, Triacylglycerols",

author = "Zeng, \{Ying Xu\} and Pedro Araujo and Du, \{Zhen Yu\} and Nguyen, \{Thu Thao\} and Livar Fr{\o}yland and Bj{\o}rn Grung",

year = "2010",

month = sep,

day = "15",

doi = "10.1016/j.talanta.2010.06.055",

language = "英语",

volume = "82",

pages = "1261--1270",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

AU - Zeng, Ying Xu

AU - Araujo, Pedro

AU - Du, Zhen Yu

AU - Nguyen, Thu Thao

AU - Frøyland, Livar

AU - Grung, Bjørn

PY - 2010/9/15

Y1 - 2010/9/15

N2 - Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS2) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS 2 for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.

AB - Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS2) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS 2 for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.

KW - Algorithm

KW - Cod liver oil

KW - Fatty acids

KW - Liquid chromatography electrospray tandem mass spectrometry

KW - Triacylglycerols

UR - https://www.scopus.com/pages/publications/77956189023

U2 - 10.1016/j.talanta.2010.06.055

DO - 10.1016/j.talanta.2010.06.055

M3 - 文章

AN - SCOPUS:77956189023

SN - 0039-9140

VL - 82

SP - 1261

EP - 1270

JO - Talanta

JF - Talanta

IS - 4

ER -

Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this