Electrogenerated chemiluminescence biosensor based on Ru(bpy)32+ and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material

Lihua Zhang; Zhiai Xu; Shaojun Dong

doi:10.1016/j.aca.2006.05.069

Electrogenerated chemiluminescence biosensor based on Ru(bpy)₃²⁺ and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material

Lihua Zhang, Zhiai Xu, Shaojun Dong

CAS - Changchun Institute of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)₃²⁺ and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)₃²⁺ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3 × 10^-6 M for alcohol (S/N = 3) with a linear range from 2.79 × 10^-5 to 5.78 × 10^-2 M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.

Original language	English
Pages (from-to)	52-56
Number of pages	5
Journal	Analytica Chimica Acta
Volume	575
Issue number	1
DOIs	https://doi.org/10.1016/j.aca.2006.05.069
State	Published - 4 Aug 2006
Externally published	Yes

Keywords

Alcohol dehydrogenase
Biosensor
Electrogenerated chemiluminescence (ECL)
Ru(bpy)
Sol-gel/chitosan/PSS

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10.1016/j.aca.2006.05.069

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title = "Electrogenerated chemiluminescence biosensor based on Ru(bpy)32+ and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material",

abstract = "A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)32+ and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)32+ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3 × 10-6 M for alcohol (S/N = 3) with a linear range from 2.79 × 10-5 to 5.78 × 10-2 M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.",

keywords = "Alcohol dehydrogenase, Biosensor, Electrogenerated chemiluminescence (ECL), Ru(bpy), Sol-gel/chitosan/PSS",

author = "Lihua Zhang and Zhiai Xu and Shaojun Dong",

year = "2006",

month = aug,

day = "4",

doi = "10.1016/j.aca.2006.05.069",

language = "英语",

volume = "575",

pages = "52--56",

journal = "Analytica Chimica Acta",

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T1 - Electrogenerated chemiluminescence biosensor based on Ru(bpy)32+ and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material

AU - Zhang, Lihua

AU - Xu, Zhiai

AU - Dong, Shaojun

PY - 2006/8/4

Y1 - 2006/8/4

N2 - A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)32+ and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)32+ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3 × 10-6 M for alcohol (S/N = 3) with a linear range from 2.79 × 10-5 to 5.78 × 10-2 M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.

AB - A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)32+ and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)32+ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3 × 10-6 M for alcohol (S/N = 3) with a linear range from 2.79 × 10-5 to 5.78 × 10-2 M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.

KW - Alcohol dehydrogenase

KW - Biosensor

KW - Electrogenerated chemiluminescence (ECL)

KW - Ru(bpy)

KW - Sol-gel/chitosan/PSS

UR - https://www.scopus.com/pages/publications/33746336245

U2 - 10.1016/j.aca.2006.05.069

DO - 10.1016/j.aca.2006.05.069

M3 - 文章

AN - SCOPUS:33746336245

SN - 0003-2670

VL - 575

SP - 52

EP - 56

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Electrogenerated chemiluminescence biosensor based on Ru(bpy)₃²⁺ and dehydrogenase immobilized in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) composite material

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