TY - JOUR
T1 - Electrode modified with toluidine blue-doped silica nanoparticles, and its use for enhanced amperometric sensing of hemoglobin
AU - Liu, Meichuan
AU - Shi, Guoyue
AU - Zhang, Li
AU - Zhao, Guohua
AU - Jin, Litong
PY - 2008/7
Y1 - 2008/7
N2 - Three-dimensionally structured, silica based, organic-inorganic hybrid nanoparticles (NPs) were prepared by a simple and feasible water-in-oil (W/O) microemulsion method and a promising platform for bioelectrochemical analysis was obtained. The commonly used phenathiazine organic compound, toluidine blue (TB) was readily captured in the three-dimensional cage of the inorganic SiO2 network, which was considered to serve as a protective "shell" toward the embedded TB. A TEM image indicated the size of the thus prepared TB-doped SiO2 (TB@SiO2) NPs was 21 ± 3 nm. UV-visible and IR spectroscopy confirmed successful formation of the organic-inorganic composite and possible interaction between TB and SiO 2, which favored enhanced stability of the hybrid. A sensitive amperometric sensor for hemoglobin (Hb) biomolecules based on TB@SiO2 NPs conjugated with a biopolymer chitosan (CHIT) membrane was then developed. The surface of the silica NPs was highly biocompatible and the TB captured inside maintained its high electron-transfer efficiency. Dye leakage of TB from the TB@SiO2 hybrid was proved to be minimal, owing to the inorganic SiO2 network and the force of interaction between TB and SiO 2. The amperometric sensor had a detection limit of 2.5 × 10-9 mol L-1 (S/N = 3) with a linear range from 5.0 × 10-9 to 3.0 × 10-6 mol L-1 for Hb. When it was applied to determine the concentration of a clinical blood sample, satisfactory results were obtained which were in good agreement with those obtained by the standard method.
AB - Three-dimensionally structured, silica based, organic-inorganic hybrid nanoparticles (NPs) were prepared by a simple and feasible water-in-oil (W/O) microemulsion method and a promising platform for bioelectrochemical analysis was obtained. The commonly used phenathiazine organic compound, toluidine blue (TB) was readily captured in the three-dimensional cage of the inorganic SiO2 network, which was considered to serve as a protective "shell" toward the embedded TB. A TEM image indicated the size of the thus prepared TB-doped SiO2 (TB@SiO2) NPs was 21 ± 3 nm. UV-visible and IR spectroscopy confirmed successful formation of the organic-inorganic composite and possible interaction between TB and SiO 2, which favored enhanced stability of the hybrid. A sensitive amperometric sensor for hemoglobin (Hb) biomolecules based on TB@SiO2 NPs conjugated with a biopolymer chitosan (CHIT) membrane was then developed. The surface of the silica NPs was highly biocompatible and the TB captured inside maintained its high electron-transfer efficiency. Dye leakage of TB from the TB@SiO2 hybrid was proved to be minimal, owing to the inorganic SiO2 network and the force of interaction between TB and SiO 2. The amperometric sensor had a detection limit of 2.5 × 10-9 mol L-1 (S/N = 3) with a linear range from 5.0 × 10-9 to 3.0 × 10-6 mol L-1 for Hb. When it was applied to determine the concentration of a clinical blood sample, satisfactory results were obtained which were in good agreement with those obtained by the standard method.
KW - Amperometric sensor
KW - Hemoglobin
KW - Toluidine blue-doped silica nanoparticles
KW - Water-in-oil microemulsion
UR - https://www.scopus.com/pages/publications/45849090746
U2 - 10.1007/s00216-008-2103-z
DO - 10.1007/s00216-008-2103-z
M3 - 文章
C2 - 18415083
AN - SCOPUS:45849090746
SN - 1618-2642
VL - 391
SP - 1951
EP - 1959
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 5
ER -