TY - JOUR
T1 - Electrochemistry-Regulated Recyclable SERS Sensor for Sensitive and Selective Detection of Tyrosinase Activity
AU - Wang, Lu
AU - Gan, Zheng Fei
AU - Guo, Dan
AU - Xia, Hai Lun
AU - Patrice, Fato Tano
AU - Hafez, Mahmoud Elsayed
AU - Li, Da Wei
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/5/21
Y1 - 2019/5/21
N2 - Tyrosinase (TYR) which can catalyze the oxidation of catechol is recognized as a significant biomarker of melanocytic lesions, thus developing powerful methods for the determination of TYR activity is highly desirable for the early diagnosis of melanin-related diseases, including melanoma. Herein, we develop a novel portable and recyclable surface-enhanced Raman scattering (SERS) sensor, prepared by assembling gold nanoparticles and p-thiol catechol (p-TC) on an ITO electrode, for detecting TYR activity via the SERS spectral variation caused by the conversion of p-TC into its corresponding quinone under TYR catalysis. The developed SERS sensor has a rapid response to TYR within 1 min under the optimized conditions and shows high selectivity for TYR with the detection limit at 0.07 U/mL. Importantly, this SERS sensor can be easily regulated by applying negative voltage to achieve circular utilization, favoring the automation of SERS detection. Furthermore, the presented recyclable SERS sensor can perform well on both the determination of TYR activity in serum and the assessment of TYR inhibitor, demonstrating huge potential in the sensitive, selective, and facile detection of TYR activity for disease diagnosis and drug screening related with TYR.
AB - Tyrosinase (TYR) which can catalyze the oxidation of catechol is recognized as a significant biomarker of melanocytic lesions, thus developing powerful methods for the determination of TYR activity is highly desirable for the early diagnosis of melanin-related diseases, including melanoma. Herein, we develop a novel portable and recyclable surface-enhanced Raman scattering (SERS) sensor, prepared by assembling gold nanoparticles and p-thiol catechol (p-TC) on an ITO electrode, for detecting TYR activity via the SERS spectral variation caused by the conversion of p-TC into its corresponding quinone under TYR catalysis. The developed SERS sensor has a rapid response to TYR within 1 min under the optimized conditions and shows high selectivity for TYR with the detection limit at 0.07 U/mL. Importantly, this SERS sensor can be easily regulated by applying negative voltage to achieve circular utilization, favoring the automation of SERS detection. Furthermore, the presented recyclable SERS sensor can perform well on both the determination of TYR activity in serum and the assessment of TYR inhibitor, demonstrating huge potential in the sensitive, selective, and facile detection of TYR activity for disease diagnosis and drug screening related with TYR.
UR - https://www.scopus.com/pages/publications/85064352688
U2 - 10.1021/acs.analchem.8b05341
DO - 10.1021/acs.analchem.8b05341
M3 - 文章
C2 - 30916930
AN - SCOPUS:85064352688
SN - 0003-2700
VL - 91
SP - 6507
EP - 6513
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -