TY - JOUR
T1 - Electrochemistry and electrocatalytic properties of hemoglobin in layer-by-layer films of SiO2 with vapor-surface sol-gel deposition
AU - Shi, Guoyue
AU - Sun, Zhiyu
AU - Liu, Meichuan
AU - Zhang, Li
AU - Liu, Ye
AU - Qu, Yunhe
AU - Jin, Litong
PY - 2007/5/15
Y1 - 2007/5/15
N2 - In this paper, layer-by-layer {Hb/SiO2}n films assembled by alternate adsorption of positively charged hemoglobin (Hb) and vapor-surface sol-gel deposition of silica at 50°C onto a glassy carbon electrode were reported. The result films were characterized with cyclic voltametery, electrochemical impedance spectroscopy, UV-vis spectroscopy, and SEM, and the direct electrochemical and electrocatalytic properties of Hb in these layer-by-layer films were investigated. A pair of well-defined quasireversible cyclic voltammetric peaks were observed, and the formal potential of the heme FeIII/FeII redox couple was found to be -0.330 V(vs SCE). The electron-transfer behavior of Hb in {Hb/SiO2}n films was dependent on the vapor temperature, the number of layers, and the pH of the Hb solution, based on which a set of optimized conditions for film fabrication was inferred. The hemoglobin in{Hb/SiO2}n films displayed good electrocatalytic activity to the reduction of hydrogen peroxide, and H 2O2 had linear current response from 1.0 × 10 -6 to 2.0 × 10-4 M with a detection limit of 5.0 × 10-7 M (S/N = 3). The apparent heterogeneous electron-transfer rate constant (ks) was 1.02 ± 0.03 s -1, and the apparent Michaeli-Menten constant (Km app) was 0.155 mM, indicating a potential application in the third-generation biosensor.
AB - In this paper, layer-by-layer {Hb/SiO2}n films assembled by alternate adsorption of positively charged hemoglobin (Hb) and vapor-surface sol-gel deposition of silica at 50°C onto a glassy carbon electrode were reported. The result films were characterized with cyclic voltametery, electrochemical impedance spectroscopy, UV-vis spectroscopy, and SEM, and the direct electrochemical and electrocatalytic properties of Hb in these layer-by-layer films were investigated. A pair of well-defined quasireversible cyclic voltammetric peaks were observed, and the formal potential of the heme FeIII/FeII redox couple was found to be -0.330 V(vs SCE). The electron-transfer behavior of Hb in {Hb/SiO2}n films was dependent on the vapor temperature, the number of layers, and the pH of the Hb solution, based on which a set of optimized conditions for film fabrication was inferred. The hemoglobin in{Hb/SiO2}n films displayed good electrocatalytic activity to the reduction of hydrogen peroxide, and H 2O2 had linear current response from 1.0 × 10 -6 to 2.0 × 10-4 M with a detection limit of 5.0 × 10-7 M (S/N = 3). The apparent heterogeneous electron-transfer rate constant (ks) was 1.02 ± 0.03 s -1, and the apparent Michaeli-Menten constant (Km app) was 0.155 mM, indicating a potential application in the third-generation biosensor.
UR - https://www.scopus.com/pages/publications/34249103582
U2 - 10.1021/ac062034g
DO - 10.1021/ac062034g
M3 - 文章
C2 - 17437331
AN - SCOPUS:34249103582
SN - 0003-2700
VL - 79
SP - 3581
EP - 3588
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -