Electrochemical immunosensor based on AuNPs/PDDA-GO for detection of SirT1

Wen Ji Bi; Dan Qin Lu; Ying Fu; Qi Lin Huang; Zhi Ai Xu; Wen Zhang

doi:10.7503/cjcu20121164

Electrochemical immunosensor based on AuNPs/PDDA-GO for detection of SirT1

Wen Ji Bi, Dan Qin Lu, Ying Fu, Qi Lin Huang, Zhi Ai Xu, Wen Zhang^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

AuNPs/PDDA-GO nanocomposite was produced by combining poly(diallyldimethylammonium chloride)(PDDA) functionalized graphene oxide nanosheets(PDDA-GO) and gold nanoparticles(AuNPs) through self-assembly method, which offered an efficient platform for antibody immobilization with good biocompatibility. A new type of electrochemical immunosensor based on AuNPs/PDDA-GO nanocomposite for the detection of SirT1 was then developed. The immunosensor construction consisted of the immobilization of the hybrid architecture, the immunoreaction of target antigen SirT1 and capture antibody(Ab₁), followed by the binding of HRP-Ab₂. The designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range for SirT1 from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.029 ng/mL. Overall, this developed strategy is proved as a simple, cost-effective method, and can be easily extended to other protein analysis.

Original language	English
Pages (from-to)	1612-1616
Number of pages	5
Journal	Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities
Volume	34
Issue number	7
DOIs	https://doi.org/10.7503/cjcu20121164
State	Published - Jul 2013

Keywords

AuNPs/PDDA-GO nanocomposite
Electrochemical immunosensor
SirT1

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title = "Electrochemical immunosensor based on AuNPs/PDDA-GO for detection of SirT1",

abstract = "AuNPs/PDDA-GO nanocomposite was produced by combining poly(diallyldimethylammonium chloride)(PDDA) functionalized graphene oxide nanosheets(PDDA-GO) and gold nanoparticles(AuNPs) through self-assembly method, which offered an efficient platform for antibody immobilization with good biocompatibility. A new type of electrochemical immunosensor based on AuNPs/PDDA-GO nanocomposite for the detection of SirT1 was then developed. The immunosensor construction consisted of the immobilization of the hybrid architecture, the immunoreaction of target antigen SirT1 and capture antibody(Ab1), followed by the binding of HRP-Ab2. The designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range for SirT1 from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.029 ng/mL. Overall, this developed strategy is proved as a simple, cost-effective method, and can be easily extended to other protein analysis.",

keywords = "AuNPs/PDDA-GO nanocomposite, Electrochemical immunosensor, SirT1",

author = "Bi, \{Wen Ji\} and Lu, \{Dan Qin\} and Ying Fu and Huang, \{Qi Lin\} and Xu, \{Zhi Ai\} and Wen Zhang",

year = "2013",

month = jul,

doi = "10.7503/cjcu20121164",

language = "英语",

volume = "34",

pages = "1612--1616",

journal = "Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities",

issn = "0251-0790",

publisher = "Higher Education Press Limited Company",

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TY - JOUR

T1 - Electrochemical immunosensor based on AuNPs/PDDA-GO for detection of SirT1

AU - Bi, Wen Ji

AU - Lu, Dan Qin

AU - Fu, Ying

AU - Huang, Qi Lin

AU - Xu, Zhi Ai

AU - Zhang, Wen

PY - 2013/7

Y1 - 2013/7

N2 - AuNPs/PDDA-GO nanocomposite was produced by combining poly(diallyldimethylammonium chloride)(PDDA) functionalized graphene oxide nanosheets(PDDA-GO) and gold nanoparticles(AuNPs) through self-assembly method, which offered an efficient platform for antibody immobilization with good biocompatibility. A new type of electrochemical immunosensor based on AuNPs/PDDA-GO nanocomposite for the detection of SirT1 was then developed. The immunosensor construction consisted of the immobilization of the hybrid architecture, the immunoreaction of target antigen SirT1 and capture antibody(Ab1), followed by the binding of HRP-Ab2. The designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range for SirT1 from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.029 ng/mL. Overall, this developed strategy is proved as a simple, cost-effective method, and can be easily extended to other protein analysis.

AB - AuNPs/PDDA-GO nanocomposite was produced by combining poly(diallyldimethylammonium chloride)(PDDA) functionalized graphene oxide nanosheets(PDDA-GO) and gold nanoparticles(AuNPs) through self-assembly method, which offered an efficient platform for antibody immobilization with good biocompatibility. A new type of electrochemical immunosensor based on AuNPs/PDDA-GO nanocomposite for the detection of SirT1 was then developed. The immunosensor construction consisted of the immobilization of the hybrid architecture, the immunoreaction of target antigen SirT1 and capture antibody(Ab1), followed by the binding of HRP-Ab2. The designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range for SirT1 from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.029 ng/mL. Overall, this developed strategy is proved as a simple, cost-effective method, and can be easily extended to other protein analysis.

KW - AuNPs/PDDA-GO nanocomposite

KW - Electrochemical immunosensor

KW - SirT1

UR - https://www.scopus.com/pages/publications/84880984786

U2 - 10.7503/cjcu20121164

DO - 10.7503/cjcu20121164

M3 - 文章

AN - SCOPUS:84880984786

SN - 0251-0790

VL - 34

SP - 1612

EP - 1616

JO - Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities

JF - Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities

IS - 7

ER -

Electrochemical immunosensor based on AuNPs/PDDA-GO for detection of SirT1

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Keywords

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