TY - JOUR
T1 - Electrochemical DNA sensor based on methylene blue functionalized polythiophene as a hybridization indicator
AU - Liu, Mengqin
AU - Luo, Chunhua
AU - Peng, Hui
PY - 2012/1/15
Y1 - 2012/1/15
N2 - A polythiophene functionalized with methylene blue (PMT-MB) was synthesized and used as an indicator for electrochemical oligonucleotides (ODNs) hybridization detection. After hybridization with complementary ODNs, the current signal of PMT-MB increased, which illustrated that PMT-MB can effectively recognize complementary ODN targets as an indicator. Compared to MB, PMT-MB showed much better resistance to the concentration change of buffer solution. In all buffer solutions tested, the hybridization always resulted in the increased current signal of PMT-MB due to the electrostatic interaction. While, when MB was used as an indicator, the inconsistent current response was obtained after the hybridization. When high concentration of buffer solution was used for accumulation, the hybridization resulted in the decreased current signal, while at the low concentrations, the current signal increased. The interaction between PMT-MB and dsODNs was also studied by UV-vis spectroscopy.
AB - A polythiophene functionalized with methylene blue (PMT-MB) was synthesized and used as an indicator for electrochemical oligonucleotides (ODNs) hybridization detection. After hybridization with complementary ODNs, the current signal of PMT-MB increased, which illustrated that PMT-MB can effectively recognize complementary ODN targets as an indicator. Compared to MB, PMT-MB showed much better resistance to the concentration change of buffer solution. In all buffer solutions tested, the hybridization always resulted in the increased current signal of PMT-MB due to the electrostatic interaction. While, when MB was used as an indicator, the inconsistent current response was obtained after the hybridization. When high concentration of buffer solution was used for accumulation, the hybridization resulted in the decreased current signal, while at the low concentrations, the current signal increased. The interaction between PMT-MB and dsODNs was also studied by UV-vis spectroscopy.
KW - Electrochemical DNA sensor
KW - Hybridization
KW - Methylene blue
KW - Polythiophene
UR - https://www.scopus.com/pages/publications/84855928410
U2 - 10.1016/j.talanta.2011.10.035
DO - 10.1016/j.talanta.2011.10.035
M3 - 文章
AN - SCOPUS:84855928410
SN - 0039-9140
VL - 88
SP - 216
EP - 221
JO - Talanta
JF - Talanta
ER -