Electrochemical biosensor for the detection of H2O2 from living cancer cells based on ZnO nanosheets

Qi Rui; Kikuo Komori; Yang Tian; Haiqing Liu; Yongping Luo; Yasuyuki Sakai

doi:10.1016/j.aca.2010.04.065

Electrochemical biosensor for the detection of H₂O₂ from living cancer cells based on ZnO nanosheets

Qi Rui
, Kikuo Komori
, Yang Tian^*
, Haiqing Liu
, Yongping Luo
, Yasuyuki Sakai

^*Corresponding author for this work

Tongji University
The University of Tokyo
Hunan University

Research output: Contribution to journal › Article › peer-review

135 Scopus citations

Abstract

In this work, direct electron transfer of cytochrome c (cyt. c)-a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E⁰') of cyt. c is estimated to be 338.2±4.3mV (vs. Ag|AgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0V (vs. Ag|AgCl), the electrochemical determination of H₂O₂ is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O₂. Such an excellent selectivity enable the present H₂O₂ biosensor determine the extracellular H₂O₂ released from living human hepatoma cells.

Original language	English
Pages (from-to)	57-62
Number of pages	6
Journal	Analytica Chimica Acta
Volume	670
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aca.2010.04.065
State	Published - Jun 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cytochrome c
Electrochemical biosensor
HO
Living cells
ZnO nanosheets

Access to Document

10.1016/j.aca.2010.04.065

Cite this

@article{0610c11a0bb6406ab85c5861dba680c2,

title = "Electrochemical biosensor for the detection of H2O2 from living cancer cells based on ZnO nanosheets",

abstract = "In this work, direct electron transfer of cytochrome c (cyt. c)-a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E0') of cyt. c is estimated to be 338.2±4.3mV (vs. Ag|AgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0V (vs. Ag|AgCl), the electrochemical determination of H2O2 is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O2. Such an excellent selectivity enable the present H2O2 biosensor determine the extracellular H2O2 released from living human hepatoma cells.",

keywords = "Cytochrome c, Electrochemical biosensor, HO, Living cells, ZnO nanosheets",

author = "Qi Rui and Kikuo Komori and Yang Tian and Haiqing Liu and Yongping Luo and Yasuyuki Sakai",

year = "2010",

month = jun,

doi = "10.1016/j.aca.2010.04.065",

language = "英语",

volume = "670",

pages = "57--62",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

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TY - JOUR

T1 - Electrochemical biosensor for the detection of H2O2 from living cancer cells based on ZnO nanosheets

AU - Rui, Qi

AU - Komori, Kikuo

AU - Tian, Yang

AU - Liu, Haiqing

AU - Luo, Yongping

AU - Sakai, Yasuyuki

PY - 2010/6

Y1 - 2010/6

N2 - In this work, direct electron transfer of cytochrome c (cyt. c)-a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E0') of cyt. c is estimated to be 338.2±4.3mV (vs. Ag|AgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0V (vs. Ag|AgCl), the electrochemical determination of H2O2 is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O2. Such an excellent selectivity enable the present H2O2 biosensor determine the extracellular H2O2 released from living human hepatoma cells.

AB - In this work, direct electron transfer of cytochrome c (cyt. c)-a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E0') of cyt. c is estimated to be 338.2±4.3mV (vs. Ag|AgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0V (vs. Ag|AgCl), the electrochemical determination of H2O2 is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O2. Such an excellent selectivity enable the present H2O2 biosensor determine the extracellular H2O2 released from living human hepatoma cells.

KW - Cytochrome c

KW - Electrochemical biosensor

KW - HO

KW - Living cells

KW - ZnO nanosheets

UR - https://www.scopus.com/pages/publications/77953807485

U2 - 10.1016/j.aca.2010.04.065

DO - 10.1016/j.aca.2010.04.065

M3 - 文章

C2 - 20685417

AN - SCOPUS:77953807485

SN - 0003-2670

VL - 670

SP - 57

EP - 62

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1-2

ER -