Electric field directed layer-by-layer assembly of horseradish peroxidase nanotubes via anodic aluminum oxide template

A novel method based on electric field directed layer-by-layer assembly (EFDLA) and template synthesis is successfully developed to fabricate horseradish peroxidase (HRP) nanotubes. It provides a rapid and general strategy for functional protein nanoarrays. The alternative deposition of poly(diallyldimethylammonium chloride) (PDDA) and HRP are characterized by SEM, TEM, UV-vis and electrochemical impedance spectroscopy (EIS). Moreover, the UV-vis spectrometry and electrochemistry of HRP indicate that immobilization of PDDA/HRP via EFDLA coupled with template synthesis could retain the conformations and bioactivities of HRP.