Effects of grazing by sheep on the structure of methane-oxidizing bacterial community of steppe soil

Xiao Qi Zhou; Yan Fen Wang; Xiang Zhong Huang; Yan Bin Hao; Jian Qing Tian; Jin Zhi Wang

doi:10.1016/j.soilbio.2007.05.020

Effects of grazing by sheep on the structure of methane-oxidizing bacterial community of steppe soil

Xiao Qi Zhou
, Yan Fen Wang^*
, Xiang Zhong Huang
, Yan Bin Hao
, Jian Qing Tian
, Jin Zhi Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Soils of three sites were studied in the Inner Mongolia steppe; one site non-grazed for 26 yr (NG26), another site non-grazed for 6 yr (NG6) and a third site freely grazed all along (FG). The composition of methantrophic communities was characterized by pmoA gene fragments (coding for a subunit of particulate methane monooxygenase) that were PCR amplified from total soil DNA extracts, using denaturing gradient gel electrophoresis (DGGE) method. Cluster analysis based on the DGGE band patterns indicated that the methanotrophic communities structure of NG6 and FG soils were similar to each other but different from that of NG26 soil. Sequence analysis showed that most bands belonged to the cluster of USCγ. This is the first report that USCγ cluster is dominated in the grassland soil.

Original language	English
Pages (from-to)	258-261
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/j.soilbio.2007.05.020
State	Published - Jan 2008
Externally published	Yes

Keywords

DGGE
Grassland
Grazing
Inner Mongolia
Methanotroph
pmoA

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10.1016/j.soilbio.2007.05.020

Cite this

@article{c79678bf341d447c9d638b201f71aa9d,

title = "Effects of grazing by sheep on the structure of methane-oxidizing bacterial community of steppe soil",

abstract = "Soils of three sites were studied in the Inner Mongolia steppe; one site non-grazed for 26 yr (NG26), another site non-grazed for 6 yr (NG6) and a third site freely grazed all along (FG). The composition of methantrophic communities was characterized by pmoA gene fragments (coding for a subunit of particulate methane monooxygenase) that were PCR amplified from total soil DNA extracts, using denaturing gradient gel electrophoresis (DGGE) method. Cluster analysis based on the DGGE band patterns indicated that the methanotrophic communities structure of NG6 and FG soils were similar to each other but different from that of NG26 soil. Sequence analysis showed that most bands belonged to the cluster of USCγ. This is the first report that USCγ cluster is dominated in the grassland soil.",

keywords = "DGGE, Grassland, Grazing, Inner Mongolia, Methanotroph, pmoA",

author = "Zhou, \{Xiao Qi\} and Wang, \{Yan Fen\} and Huang, \{Xiang Zhong\} and Hao, \{Yan Bin\} and Tian, \{Jian Qing\} and Wang, \{Jin Zhi\}",

year = "2008",

month = jan,

doi = "10.1016/j.soilbio.2007.05.020",

language = "英语",

volume = "40",

pages = "258--261",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Effects of grazing by sheep on the structure of methane-oxidizing bacterial community of steppe soil

AU - Zhou, Xiao Qi

AU - Wang, Yan Fen

AU - Huang, Xiang Zhong

AU - Hao, Yan Bin

AU - Tian, Jian Qing

AU - Wang, Jin Zhi

PY - 2008/1

Y1 - 2008/1

N2 - Soils of three sites were studied in the Inner Mongolia steppe; one site non-grazed for 26 yr (NG26), another site non-grazed for 6 yr (NG6) and a third site freely grazed all along (FG). The composition of methantrophic communities was characterized by pmoA gene fragments (coding for a subunit of particulate methane monooxygenase) that were PCR amplified from total soil DNA extracts, using denaturing gradient gel electrophoresis (DGGE) method. Cluster analysis based on the DGGE band patterns indicated that the methanotrophic communities structure of NG6 and FG soils were similar to each other but different from that of NG26 soil. Sequence analysis showed that most bands belonged to the cluster of USCγ. This is the first report that USCγ cluster is dominated in the grassland soil.

AB - Soils of three sites were studied in the Inner Mongolia steppe; one site non-grazed for 26 yr (NG26), another site non-grazed for 6 yr (NG6) and a third site freely grazed all along (FG). The composition of methantrophic communities was characterized by pmoA gene fragments (coding for a subunit of particulate methane monooxygenase) that were PCR amplified from total soil DNA extracts, using denaturing gradient gel electrophoresis (DGGE) method. Cluster analysis based on the DGGE band patterns indicated that the methanotrophic communities structure of NG6 and FG soils were similar to each other but different from that of NG26 soil. Sequence analysis showed that most bands belonged to the cluster of USCγ. This is the first report that USCγ cluster is dominated in the grassland soil.

KW - DGGE

KW - Grassland

KW - Grazing

KW - Inner Mongolia

KW - Methanotroph

KW - pmoA

UR - https://www.scopus.com/pages/publications/35348919478

U2 - 10.1016/j.soilbio.2007.05.020

DO - 10.1016/j.soilbio.2007.05.020

M3 - 文章

AN - SCOPUS:35348919478

SN - 0038-0717

VL - 40

SP - 258

EP - 261

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

IS - 1

ER -

Effects of grazing by sheep on the structure of methane-oxidizing bacterial community of steppe soil

Abstract

Keywords

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