Effects of exercise training on β-amyloid protein 42 and β-amyloid precursor protein cleaving enzyme 1 in hippocampus of D-galactose induced Alzheimer's disease model rats

Objective: To study the effects of swimming and treadmill training on hippocampal β-amyloid precursor protein 42(Aβ42) and it's cleaving enzymel(BACEl) in rat model of D-galactose induced Alzheimer's disease (AD). Method: Sixteen SD male rats were randomly divided into sedentary injection group (C) and exercise injection group (T). All of the rats were received injection of D-galactose into abdominal cavity continuously for 8 weeks to make AD animal models. Group T were in addition given mixed training (swimming and treadmill training alternatively each 3 times per week) for 8 weeks. The levels of hippocampal Aβ42 protein of rats were inspected by Western blot method. The expression levels of hippocampal BACE1 mRNA and insulin derived growth factory (IGF)-1mRNA of rats were inspected by RT-PCR technique. Result:(1)Compared with group C, expression of Aβ42 protein in group T reduced significantly in hippocampus of rats(P<0.01). (2)Expres sion of BACE1 mRNA was higher in group C than that in group T (P<0.05). (3)Compared with group C, up-regulation of expression of hippocampal IGF-lmRNA in group T was founded (P<0.01). Conclusion: This investigation demonstrates that swimming and treadmill training mixed exercise is a simple behavioral intervention, can inhibit the progression of AD. It may be related with the up-regulation of expression of hippocampal IGF-1mRNA by exercise training.