Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH

Yufeng Yuan; Kehan Huang; Mengfang Chang; Cuifang Qin; Sanjun Zhang; Haifeng Pan; Yan Chen; Jianhua Xu

doi:10.1016/j.ab.2015.09.021

Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH

Yufeng Yuan, Kehan Huang, Mengfang Chang, Cuifang Qin, Sanjun Zhang, Haifeng Pan, Yan Chen, Jianhua Xu

Institute for Advanced Study in Precision Spectroscopy

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD⁺ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD⁺ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD⁺ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I₃₉₅/I₅₅₀), fitting by a single-exponential decay function, can efficiently detect various NAD⁺ levels from 100 to 4000 μM, as well as label NAD⁺/NADH (reduced form of NAD) ratios in the range of 1-50.

Original language	English
Pages (from-to)	46-48
Number of pages	3
Journal	Analytical Biochemistry
Volume	494
DOIs	https://doi.org/10.1016/j.ab.2015.09.021
State	Published - 1 Feb 2016

Keywords

Biological coenzyme NAD
Dual-emission fluorescent silver nanoclusters
Dual-emission intensity ratio
NAD/NADH ratios

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10.1016/j.ab.2015.09.021

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title = "Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH",

abstract = "Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD+ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD+ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD+ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD+ levels from 100 to 4000 μM, as well as label NAD+/NADH (reduced form of NAD) ratios in the range of 1-50.",

keywords = "Biological coenzyme NAD, Dual-emission fluorescent silver nanoclusters, Dual-emission intensity ratio, NAD/NADH ratios",

author = "Yufeng Yuan and Kehan Huang and Mengfang Chang and Cuifang Qin and Sanjun Zhang and Haifeng Pan and Yan Chen and Jianhua Xu",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

year = "2016",

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day = "1",

doi = "10.1016/j.ab.2015.09.021",

language = "英语",

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journal = "Analytical Biochemistry",

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TY - JOUR

T1 - Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH

AU - Yuan, Yufeng

AU - Huang, Kehan

AU - Chang, Mengfang

AU - Qin, Cuifang

AU - Zhang, Sanjun

AU - Pan, Haifeng

AU - Chen, Yan

AU - Xu, Jianhua

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD+ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD+ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD+ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD+ levels from 100 to 4000 μM, as well as label NAD+/NADH (reduced form of NAD) ratios in the range of 1-50.

AB - Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD+ (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD+ concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD+ and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD+ levels from 100 to 4000 μM, as well as label NAD+/NADH (reduced form of NAD) ratios in the range of 1-50.

KW - Biological coenzyme NAD

KW - Dual-emission fluorescent silver nanoclusters

KW - Dual-emission intensity ratio

KW - NAD/NADH ratios

UR - https://www.scopus.com/pages/publications/84948442632

U2 - 10.1016/j.ab.2015.09.021

DO - 10.1016/j.ab.2015.09.021

M3 - 文章

C2 - 26452612

AN - SCOPUS:84948442632

SN - 0003-2697

VL - 494

SP - 46

EP - 48

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH

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Keywords

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