Dual base editor catalyzes both cytosine and adenine base conversions in human cells

Xiaohui Zhang; Biyun Zhu; Liang Chen; Ling Xie; Weishi Yu; Ying Wang; Linxi Li; Shuming Yin; Lei Yang; Handan Hu; Honghui Han; Yongmei Li; Liren Wang; Geng Chen; Xueyun Ma; Hongquan Geng; Wanfeng Huang; Xiufeng Pang; Zuozhen Yang; Yuxuan Wu; Stefan Siwko; Ryo Kurita; Yukio Nakamura; Li Yang; Mingyao Liu; Dali Li

doi:10.1038/s41587-020-0527-y

Dual base editor catalyzes both cytosine and adenine base conversions in human cells

Xiaohui Zhang, Biyun Zhu, Liang Chen, Ling Xie, Weishi Yu, Ying Wang, Linxi Li, Shuming Yin, Lei Yang, Handan Hu, Honghui Han, Yongmei Li, Liren Wang, Geng Chen, Xueyun Ma, Hongquan Geng, Wanfeng Huang, Xiufeng Pang, Zuozhen Yang, Yuxuan WuStefan Siwko, Ryo Kurita, Yukio Nakamura, Li Yang, Mingyao Liu, Dali Li^*

^*Corresponding author for this work

School of Life Sciences

Research output: Contribution to journal › Article › peer-review

234 Scopus citations

Abstract

Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax’s activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.

Original language	English
Pages (from-to)	856-860
Number of pages	5
Journal	Nature Biotechnology
Volume	38
Issue number	7
DOIs	https://doi.org/10.1038/s41587-020-0527-y
State	Published - 1 Jul 2020

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title = "Dual base editor catalyzes both cytosine and adenine base conversions in human cells",

abstract = "Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A\&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A\&C-BEmax{\textquoteright}s activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.",

author = "Xiaohui Zhang and Biyun Zhu and Liang Chen and Ling Xie and Weishi Yu and Ying Wang and Linxi Li and Shuming Yin and Lei Yang and Handan Hu and Honghui Han and Yongmei Li and Liren Wang and Geng Chen and Xueyun Ma and Hongquan Geng and Wanfeng Huang and Xiufeng Pang and Zuozhen Yang and Yuxuan Wu and Stefan Siwko and Ryo Kurita and Yukio Nakamura and Li Yang and Mingyao Liu and Dali Li",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2020",

month = jul,

day = "1",

doi = "10.1038/s41587-020-0527-y",

language = "英语",

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Zhang, X, Zhu, B, Chen, L, Xie, L, Yu, W, Wang, Y, Li, L, Yin, S, Yang, L, Hu, H, Han, H, Li, Y, Wang, L, Chen, G, Ma, X, Geng, H, Huang, W, Pang, X, Yang, Z, Wu, Y, Siwko, S, Kurita, R, Nakamura, Y, Yang, L, Liu, M & Li, D 2020, 'Dual base editor catalyzes both cytosine and adenine base conversions in human cells', Nature Biotechnology, vol. 38, no. 7, pp. 856-860. https://doi.org/10.1038/s41587-020-0527-y

TY - JOUR

T1 - Dual base editor catalyzes both cytosine and adenine base conversions in human cells

AU - Zhang, Xiaohui

AU - Zhu, Biyun

AU - Chen, Liang

AU - Xie, Ling

AU - Yu, Weishi

AU - Wang, Ying

AU - Li, Linxi

AU - Yin, Shuming

AU - Yang, Lei

AU - Hu, Handan

AU - Han, Honghui

AU - Li, Yongmei

AU - Wang, Liren

AU - Chen, Geng

AU - Ma, Xueyun

AU - Geng, Hongquan

AU - Huang, Wanfeng

AU - Pang, Xiufeng

AU - Yang, Zuozhen

AU - Wu, Yuxuan

AU - Siwko, Stefan

AU - Kurita, Ryo

AU - Nakamura, Yukio

AU - Yang, Li

AU - Liu, Mingyao

AU - Li, Dali

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax’s activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.

AB - Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax’s activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.

UR - https://www.scopus.com/pages/publications/85085875296

U2 - 10.1038/s41587-020-0527-y

DO - 10.1038/s41587-020-0527-y

M3 - 文章

C2 - 32483363

AN - SCOPUS:85085875296

SN - 1087-0156

VL - 38

SP - 856

EP - 860

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 7

ER -

Dual base editor catalyzes both cytosine and adenine base conversions in human cells

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