TY - JOUR
T1 - DNA Tetrahedral Nanostructure-Based Electrochemical miRNA Biosensor for Simultaneous Detection of Multiple miRNAs in Pancreatic Carcinoma
AU - Zeng, Dongdong
AU - Wang, Zehua
AU - Meng, Zhiqiang
AU - Wang, Peng
AU - San, Lili
AU - Wang, Wei
AU - Aldalbahi, Ali
AU - Li, Li
AU - Shen, Juwen
AU - Mi, Xianqiang
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/7/19
Y1 - 2017/7/19
N2 - Specific and sensitive biomarker detection is essential to early cancer diagnosis. In this study, we demonstrate an ultrasensitive electrochemical biosensor with the ability to detect multiple pancreatic carcinoma (PC)-related microRNA biomarkers. By employing DNA tetrahedral nanostructure capture probes to enhance the detection sensitivity as well as a disposable 16-channel screen-printed gold electrode (SPGE) detection platform to enhance the detection efficiency, we were able to simultaneously detect four PC-related miRNAs: miRNA21, miRNA155, miRNA196a, and miRNA210. The detection sensitivity reached to as low as 10 fM. We then profiled the serum levels of the four miRNAs for PC patients and healthy individuals with our multiplexing electrochemical biosensor. Through the combined analyses of the four miRNAs, our results showed that PC patients could be discriminated from healthy controls with fairly high sensitivity. This multiplexing PCR-free miRNA detection sensor shows promising applications in early diagnosis of PC disease.
AB - Specific and sensitive biomarker detection is essential to early cancer diagnosis. In this study, we demonstrate an ultrasensitive electrochemical biosensor with the ability to detect multiple pancreatic carcinoma (PC)-related microRNA biomarkers. By employing DNA tetrahedral nanostructure capture probes to enhance the detection sensitivity as well as a disposable 16-channel screen-printed gold electrode (SPGE) detection platform to enhance the detection efficiency, we were able to simultaneously detect four PC-related miRNAs: miRNA21, miRNA155, miRNA196a, and miRNA210. The detection sensitivity reached to as low as 10 fM. We then profiled the serum levels of the four miRNAs for PC patients and healthy individuals with our multiplexing electrochemical biosensor. Through the combined analyses of the four miRNAs, our results showed that PC patients could be discriminated from healthy controls with fairly high sensitivity. This multiplexing PCR-free miRNA detection sensor shows promising applications in early diagnosis of PC disease.
KW - DNA tetrahedral nanostructures
KW - biomarker
KW - electrochemical biosensor
KW - multiple miRNA detection
KW - pancreatic carcinoma
UR - https://www.scopus.com/pages/publications/85024888013
U2 - 10.1021/acsami.7b05981
DO - 10.1021/acsami.7b05981
M3 - 文章
C2 - 28660759
AN - SCOPUS:85024888013
SN - 1944-8244
VL - 9
SP - 24118
EP - 24125
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 28
ER -
