DNA separation by microchip electrophoresis using copolymers of poly(vinylpyrrolidone) and hydroxyethylcellulose

Gang Li; Shuli Ge; Xiaofang Ni; Shuqing Dong; Qingjiang Wang; Pingang He; Yuzhi Fang

doi:10.1002/cjoc.200990370

DNA separation by microchip electrophoresis using copolymers of poly(vinylpyrrolidone) and hydroxyethylcellulose

Gang Li
, Shuli Ge
, Xiaofang Ni
, Shuqing Dong
, Qingjiang Wang
, Pingang He
, Yuzhi Fang

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Copolymers of poly(vinylpyrrolidone) (PVP) and hydroxyethylcellulose (HEC) were synthesized, with PVP to HEC molar ratios of 3: 1, 2: 1 and 1: 1. The copolymers were tested as separation media in DNA fragment separation analysis by microchip electrophoresis (MCE). Separation efficiency over 3.8×10⁵ for 118 bp has been reached by using the bare channels without the additional polymer coating step. Under optimized separation conditions for longer read length DNA sequencing, the separation ability of the copolymers decreased with decreasing (PVP-co-HEC) molar ratio from 3: 1 to 2: 1 and 1: 1. In comparison with (PVP-co-HEC) 1: 1, the copolymer with (PVP-co-HEC) 3: 1 ratio showed high separation efficiency. By using a 20 g•L^-1 copolymer with (PVP-co-HEC) 3: 1 ratio, ΦΧ174-HaeIII digest DNA marker was successfully separated within 3 min.

Original language	English
Pages (from-to)	2207-2211
Number of pages	5
Journal	Chinese Journal of Chemistry
Volume	27
Issue number	11
DOIs	https://doi.org/10.1002/cjoc.200990370
State	Published - Nov 2009

Keywords

Copolymer
DNA separation
Microfluidics

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10.1002/cjoc.200990370

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title = "DNA separation by microchip electrophoresis using copolymers of poly(vinylpyrrolidone) and hydroxyethylcellulose",

abstract = "Copolymers of poly(vinylpyrrolidone) (PVP) and hydroxyethylcellulose (HEC) were synthesized, with PVP to HEC molar ratios of 3: 1, 2: 1 and 1: 1. The copolymers were tested as separation media in DNA fragment separation analysis by microchip electrophoresis (MCE). Separation efficiency over 3.8×105 for 118 bp has been reached by using the bare channels without the additional polymer coating step. Under optimized separation conditions for longer read length DNA sequencing, the separation ability of the copolymers decreased with decreasing (PVP-co-HEC) molar ratio from 3: 1 to 2: 1 and 1: 1. In comparison with (PVP-co-HEC) 1: 1, the copolymer with (PVP-co-HEC) 3: 1 ratio showed high separation efficiency. By using a 20 g•L-1 copolymer with (PVP-co-HEC) 3: 1 ratio, ΦΧ174-HaeIII digest DNA marker was successfully separated within 3 min.",

keywords = "Copolymer, DNA separation, Microfluidics",

author = "Gang Li and Shuli Ge and Xiaofang Ni and Shuqing Dong and Qingjiang Wang and Pingang He and Yuzhi Fang",

year = "2009",

month = nov,

doi = "10.1002/cjoc.200990370",

language = "英语",

volume = "27",

pages = "2207--2211",

journal = "Chinese Journal of Chemistry",

issn = "1001-604X",

publisher = "John Wiley and Sons Inc",

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}

TY - JOUR

T1 - DNA separation by microchip electrophoresis using copolymers of poly(vinylpyrrolidone) and hydroxyethylcellulose

AU - Li, Gang

AU - Ge, Shuli

AU - Ni, Xiaofang

AU - Dong, Shuqing

AU - Wang, Qingjiang

AU - He, Pingang

AU - Fang, Yuzhi

PY - 2009/11

Y1 - 2009/11

N2 - Copolymers of poly(vinylpyrrolidone) (PVP) and hydroxyethylcellulose (HEC) were synthesized, with PVP to HEC molar ratios of 3: 1, 2: 1 and 1: 1. The copolymers were tested as separation media in DNA fragment separation analysis by microchip electrophoresis (MCE). Separation efficiency over 3.8×105 for 118 bp has been reached by using the bare channels without the additional polymer coating step. Under optimized separation conditions for longer read length DNA sequencing, the separation ability of the copolymers decreased with decreasing (PVP-co-HEC) molar ratio from 3: 1 to 2: 1 and 1: 1. In comparison with (PVP-co-HEC) 1: 1, the copolymer with (PVP-co-HEC) 3: 1 ratio showed high separation efficiency. By using a 20 g•L-1 copolymer with (PVP-co-HEC) 3: 1 ratio, ΦΧ174-HaeIII digest DNA marker was successfully separated within 3 min.

AB - Copolymers of poly(vinylpyrrolidone) (PVP) and hydroxyethylcellulose (HEC) were synthesized, with PVP to HEC molar ratios of 3: 1, 2: 1 and 1: 1. The copolymers were tested as separation media in DNA fragment separation analysis by microchip electrophoresis (MCE). Separation efficiency over 3.8×105 for 118 bp has been reached by using the bare channels without the additional polymer coating step. Under optimized separation conditions for longer read length DNA sequencing, the separation ability of the copolymers decreased with decreasing (PVP-co-HEC) molar ratio from 3: 1 to 2: 1 and 1: 1. In comparison with (PVP-co-HEC) 1: 1, the copolymer with (PVP-co-HEC) 3: 1 ratio showed high separation efficiency. By using a 20 g•L-1 copolymer with (PVP-co-HEC) 3: 1 ratio, ΦΧ174-HaeIII digest DNA marker was successfully separated within 3 min.

KW - Copolymer

KW - DNA separation

KW - Microfluidics

UR - https://www.scopus.com/pages/publications/77749334741

U2 - 10.1002/cjoc.200990370

DO - 10.1002/cjoc.200990370

M3 - 文章

AN - SCOPUS:77749334741

SN - 1001-604X

VL - 27

SP - 2207

EP - 2211

JO - Chinese Journal of Chemistry

JF - Chinese Journal of Chemistry

IS - 11

ER -

DNA separation by microchip electrophoresis using copolymers of poly(vinylpyrrolidone) and hydroxyethylcellulose

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Keywords

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