DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA

Hui Peng; Lijuan Zhang; Tanja H.M. Kjällman; Christian Soeller; Jadranka Travas-Sejdic

doi:10.1021/ja0685452

DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA

Hui Peng, Lijuan Zhang, Tanja H.M. Kjällman, Christian Soeller, Jadranka Travas-Sejdic

The University of Auckland

Research output: Contribution to journal › Article › peer-review

270 Scopus citations

Abstract

A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.

Original language	English
Pages (from-to)	3048-3049
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	11
DOIs	https://doi.org/10.1021/ja0685452
State	Published - 21 Mar 2007
Externally published	Yes

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AU - Zhang, Lijuan

AU - Kjällman, Tanja H.M.

AU - Soeller, Christian

AU - Travas-Sejdic, Jadranka

PY - 2007/3/21

Y1 - 2007/3/21

N2 - A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.

AB - A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.

UR - https://www.scopus.com/pages/publications/33947361508

U2 - 10.1021/ja0685452

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M3 - 文章

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ER -

DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA

Abstract

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