DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition

Xiao Qin Jiang; Su Miao Guo; Min Zhang; Ming Zhou; Bang Ce Ye

doi:10.1039/c4an01373e

DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition

Xiao Qin Jiang
, Su Miao Guo
, Min Zhang^*
, Ming Zhou
, Bang Ce Ye

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China University of Science and Technology
University of Washington

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

Original language	English
Pages (from-to)	5682-5685
Number of pages	4
Journal	Analyst
Volume	139
Issue number	22
DOIs	https://doi.org/10.1039/c4an01373e
State	Published - 15 Oct 2014

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@article{4626f2aadaf141ce887c664500409d0f,

title = "DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition",

abstract = "A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.",

author = "Jiang, \{Xiao Qin\} and Guo, \{Su Miao\} and Min Zhang and Ming Zhou and Ye, \{Bang Ce\}",

year = "2014",

month = oct,

day = "15",

doi = "10.1039/c4an01373e",

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volume = "139",

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TY - JOUR

T1 - DNA-hosted Hoechst dyes

T2 - Application for label-free fluorescent monitoring of endonuclease activity and inhibition

AU - Jiang, Xiao Qin

AU - Guo, Su Miao

AU - Zhang, Min

AU - Zhou, Ming

AU - Ye, Bang Ce

PY - 2014/10/15

Y1 - 2014/10/15

N2 - A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

AB - A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

UR - https://www.scopus.com/pages/publications/84907989518

U2 - 10.1039/c4an01373e

DO - 10.1039/c4an01373e

M3 - 文章

C2 - 25272021

AN - SCOPUS:84907989518

SN - 0003-2654

VL - 139

SP - 5682

EP - 5685

JO - Analyst

JF - Analyst

IS - 22

ER -

DNA-hosted Hoechst dyes: Application for label-free fluorescent monitoring of endonuclease activity and inhibition

Abstract

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