Dispersal limitation drives biogeographical patterns of anammox bacterial communities across the Yangtze River

Over the past few decades, anaerobic ammonium oxidation (anammox) has been extensively documented at different scales in natural ecosystems. Previous studies have stated that the community composition of anammox bacteria is shaped mainly by environmental factors, whereas spatial factors have been largely overlooked. This study investigated biogeographical patterns of anammox bacterial communities using 42 sediment samples along a 4300-km stretch of the Yangtze River, the longest river in Asia. A significant distance-decay relationship was observed for anammox bacterial community similarity, which was significantly influenced by mean dendritic distance rather than environmental factors. This implied that dispersal limitation plays an important role in shaping biogeographical pattern of anammox community. Furthermore, our results revealed that neutral processes played vital role in shaping community assembly of anammox bacteria, and their communities were seriously dispersal limited. These findings contrast with previous studies on community similarities between broad taxonomic groups, which are mainly determined by niche-based selection owing to greater niche distances within broad taxonomic groups than in anammox bacteria. Importantly, the slope of the distance-decay curve was much steeper than previously reported for whole bacteria, which indicating the species turnover rate of anammox bacterial community was significantly higher than that of the whole bacterial community. Anammox bacteria harbor strong adhesion ability and low dispersal potential, and ultimately exhibited a high species turnover rate. Together, in the context of biogeography, our results highlight the importance of dispersal limitation in shaping the biogeographical pattern of anammox bacterial community.