Direct monitoring of nitric oxide release from fibrocytes with PVP/Pd/IrO₂/nafion chemically modified microelectrode

PVP/Pd/IrO₂/Nafion microsensor was fabricated to measure nitric oxide production in fibrocytes directly. In the experiments, fibrocytes did not release nitric oxide after stimulated with L-arginine and acetylcholine under normal condition. After induced with lipopolysaccharide, NO was produced in high concentration and NO levels were increased after stimulated with L-arginine and acetylcholine. On the other hand, L-Nω-nitro-arginine, an NO synthase inhibitor, decreased the action of L-arginine and acetylcholine.