TY - JOUR
T1 - Direct electrochemistry of superoxide dismutases (Mn-, Fe-, and Ni-) from human pathogen Clostridium difficile
T2 - Toward application to superoxide biosensor
AU - Ye, Qing
AU - Li, Wei
AU - Wang, Zhen
AU - Zhang, Limin
AU - Tan, Xiangshi
AU - Tian, Yang
PY - 2014/9/1
Y1 - 2014/9/1
N2 - In this work, direct electrochemistry and electrocatalytic activity of four new kinds of artificially prepared superoxide dismutases (SODs) from human pathogen Clostridium difficile with different metal centers and microenvironments, that is, manganese superoxide dismutase (Mn-SOD), azide ion-coordinated manganese superoxide dismutase (Mn(N3 -)-SOD), iron superoxide dismutase (Fe-SOD), and nickel superoxide dismutase (Ni-SOD), are systematically investigated. The direct electron transfer of the SODs is realized by 3-mercaptopropionic acid (MPA) confined on gold flowers electrodeposited onto glassy carbon electrode. The electrochemical parameters are dependent on the metal centers and microenvironments of the SODs with respect to the formal potential, reversibility of electrode reactions, and kinetic rate constant. Furthermore, except Ni-SOD, the other three kinds of SODs all exhibit a bifunctional electrocatalytic activity toward O2 -. The combination of the enhanced electron transfer and the enzyme catalytic activities of SODs enables a sensitive and selective determination of O2-.
AB - In this work, direct electrochemistry and electrocatalytic activity of four new kinds of artificially prepared superoxide dismutases (SODs) from human pathogen Clostridium difficile with different metal centers and microenvironments, that is, manganese superoxide dismutase (Mn-SOD), azide ion-coordinated manganese superoxide dismutase (Mn(N3 -)-SOD), iron superoxide dismutase (Fe-SOD), and nickel superoxide dismutase (Ni-SOD), are systematically investigated. The direct electron transfer of the SODs is realized by 3-mercaptopropionic acid (MPA) confined on gold flowers electrodeposited onto glassy carbon electrode. The electrochemical parameters are dependent on the metal centers and microenvironments of the SODs with respect to the formal potential, reversibility of electrode reactions, and kinetic rate constant. Furthermore, except Ni-SOD, the other three kinds of SODs all exhibit a bifunctional electrocatalytic activity toward O2 -. The combination of the enhanced electron transfer and the enzyme catalytic activities of SODs enables a sensitive and selective determination of O2-.
KW - Direct electron transfer
KW - Electrocatalytic activity
KW - Electrochemical parameters
KW - Superoxide anion
KW - Superoxide dismutase
UR - https://www.scopus.com/pages/publications/84905389050
U2 - 10.1016/j.jelechem.2014.06.029
DO - 10.1016/j.jelechem.2014.06.029
M3 - 文章
AN - SCOPUS:84905389050
SN - 1572-6657
VL - 729
SP - 21
EP - 26
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
ER -