Abstract
Objective: To study the differentiation-inducing effect of hydroxycamptothecin (HCPT) on hepatoma Hep G2 cells. Methods: 3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyltetrazolinium bromide (MTT) colorimetric method was used for determining HepG2 cells viability. Alphafetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits. Results: The AFP secretion of HCPT-treated cells was reduced by 44.0%-49.2% while the ALB level was increased markedly. Conclusion: Hydroxycamptothecin can induce human hepatoma Hep G2 cells to differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 712-713 |
| Number of pages | 2 |
| Journal | Chinese Medical Journal |
| Volume | 113 |
| Issue number | 8 |
| State | Published - Aug 2000 |
| Externally published | Yes |
Keywords
- Differentiation
- Hepatoma cells
- Hydroxycamptothecin