Different oxidants and PKC isozymes mediate the opposite effect of inhibition of Qi and Qo site of mitochondrial complex III on calcium currents in rat cortical neurons

Pei Ying Wu; Bin Lai; Yi Dong; Ze Min Wang; Zi Cheng Li; Ping Zheng

doi:10.1016/j.bbamcr.2010.05.001

Different oxidants and PKC isozymes mediate the opposite effect of inhibition of Q_i and Q_o site of mitochondrial complex III on calcium currents in rat cortical neurons

Pei Ying Wu
, Bin Lai
, Yi Dong
, Ze Min Wang
, Zi Cheng Li
, Ping Zheng^*

^*Corresponding author for this work

Fudan University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The inhibition of the complex III of the mitochondrial respiratory chain under hypoxia-ischemia has been observed. However, the downstream events of this inhibition remain to be studied. In this paper, we used the Q_i site inhibitor antimycin A and the Q_o site inhibitor myxothiazol to inhibit the Q_i site and the Q_o site of the complex III and studied the effect and mechanism of the inhibition of these sites on voltage-gated Ca²⁺ currents (I_Ca) in rat prefrontal neurons with whole cell patch-clamp method in slices. The results showed that antimycin A inhibited I_Ca, but myxothiazol increased it. Further mechanism study showed that antimycin A inhibited I_Ca via the H₂O₂-hydroxyl radicals/cPKC (mainly PKCβI) pathway, whereas myxothiazol increased I_Ca via the superoxide anion/nPKC (mainly the PKCδ) pathway.

Original language	English
Pages (from-to)	1072-1082
Number of pages	11
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1803
Issue number	9
DOIs	https://doi.org/10.1016/j.bbamcr.2010.05.001
State	Published - Sep 2010
Externally published	Yes

Keywords

Calcium currents
Cortical neurons
Mitochondrial complex III
Protein kinase C
Reactive oxygen species
Whole-cell patch-clamp

Access to Document

10.1016/j.bbamcr.2010.05.001

Cite this

@article{8d5b21b7338944bd88991d9bde265f3a,

title = "Different oxidants and PKC isozymes mediate the opposite effect of inhibition of Qi and Qo site of mitochondrial complex III on calcium currents in rat cortical neurons",

abstract = "The inhibition of the complex III of the mitochondrial respiratory chain under hypoxia-ischemia has been observed. However, the downstream events of this inhibition remain to be studied. In this paper, we used the Qi site inhibitor antimycin A and the Qo site inhibitor myxothiazol to inhibit the Qi site and the Qo site of the complex III and studied the effect and mechanism of the inhibition of these sites on voltage-gated Ca2+ currents (ICa) in rat prefrontal neurons with whole cell patch-clamp method in slices. The results showed that antimycin A inhibited ICa, but myxothiazol increased it. Further mechanism study showed that antimycin A inhibited ICa via the H2O2-hydroxyl radicals/cPKC (mainly PKCβI) pathway, whereas myxothiazol increased ICa via the superoxide anion/nPKC (mainly the PKCδ) pathway.",

keywords = "Calcium currents, Cortical neurons, Mitochondrial complex III, Protein kinase C, Reactive oxygen species, Whole-cell patch-clamp",

author = "Wu, \{Pei Ying\} and Bin Lai and Yi Dong and Wang, \{Ze Min\} and Li, \{Zi Cheng\} and Ping Zheng",

year = "2010",

month = sep,

doi = "10.1016/j.bbamcr.2010.05.001",

language = "英语",

volume = "1803",

pages = "1072--1082",

journal = "Biochimica et Biophysica Acta - Molecular Cell Research",

issn = "0167-4889",

publisher = "Elsevier B.V.",

number = "9",

}

Different oxidants and PKC isozymes mediate the opposite effect of inhibition of Q_i and Q_o site of mitochondrial complex III on calcium currents in rat cortical neurons. / Wu, Pei Ying; Lai, Bin; Dong, Yi et al.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1803, No. 9, 09.2010, p. 1072-1082.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Different oxidants and PKC isozymes mediate the opposite effect of inhibition of Qi and Qo site of mitochondrial complex III on calcium currents in rat cortical neurons

AU - Wu, Pei Ying

AU - Lai, Bin

AU - Dong, Yi

AU - Wang, Ze Min

AU - Li, Zi Cheng

AU - Zheng, Ping

PY - 2010/9

Y1 - 2010/9

N2 - The inhibition of the complex III of the mitochondrial respiratory chain under hypoxia-ischemia has been observed. However, the downstream events of this inhibition remain to be studied. In this paper, we used the Qi site inhibitor antimycin A and the Qo site inhibitor myxothiazol to inhibit the Qi site and the Qo site of the complex III and studied the effect and mechanism of the inhibition of these sites on voltage-gated Ca2+ currents (ICa) in rat prefrontal neurons with whole cell patch-clamp method in slices. The results showed that antimycin A inhibited ICa, but myxothiazol increased it. Further mechanism study showed that antimycin A inhibited ICa via the H2O2-hydroxyl radicals/cPKC (mainly PKCβI) pathway, whereas myxothiazol increased ICa via the superoxide anion/nPKC (mainly the PKCδ) pathway.

AB - The inhibition of the complex III of the mitochondrial respiratory chain under hypoxia-ischemia has been observed. However, the downstream events of this inhibition remain to be studied. In this paper, we used the Qi site inhibitor antimycin A and the Qo site inhibitor myxothiazol to inhibit the Qi site and the Qo site of the complex III and studied the effect and mechanism of the inhibition of these sites on voltage-gated Ca2+ currents (ICa) in rat prefrontal neurons with whole cell patch-clamp method in slices. The results showed that antimycin A inhibited ICa, but myxothiazol increased it. Further mechanism study showed that antimycin A inhibited ICa via the H2O2-hydroxyl radicals/cPKC (mainly PKCβI) pathway, whereas myxothiazol increased ICa via the superoxide anion/nPKC (mainly the PKCδ) pathway.

KW - Calcium currents

KW - Cortical neurons

KW - Mitochondrial complex III

KW - Protein kinase C

KW - Reactive oxygen species

KW - Whole-cell patch-clamp

UR - https://www.scopus.com/pages/publications/77954953997

U2 - 10.1016/j.bbamcr.2010.05.001

DO - 10.1016/j.bbamcr.2010.05.001

M3 - 文章

C2 - 20488213

AN - SCOPUS:77954953997

SN - 0167-4889

VL - 1803

SP - 1072

EP - 1082

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

IS - 9

ER -

Different oxidants and PKC isozymes mediate the opposite effect of inhibition of Q_i and Q_o site of mitochondrial complex III on calcium currents in rat cortical neurons

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this