TY - JOUR
T1 - Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe
AU - Jin, Shengye
AU - Xu, Zhaochao
AU - Chen, Jiping
AU - Liang, Xinmiao
AU - Wu, Yongning
AU - Qian, Xuhong
PY - 2004/10/4
Y1 - 2004/10/4
N2 - A flow injection system for the determination of organophosphate and carbamate pesticides is described. A sensitive fluorescence probe was synthesized and used as the pH indicator to detect the inhibition of the enzyme acetylcholinesterase (AChE). The percentage inhibition of enzyme activity is correlated to the pesticide concentration. Several parameters influencing the performance of the system are discussed. The detection limits of 3.5, 50, 12 and 25 μg/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. A complete cycle of analysis, including incubation time, took 14 min. The detection system has been applied to the determination of carbofuran in spiked vegetable juices (Chinese cabbage and cole), achieving recovery values between 93.2 and 107% for Chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed.
AB - A flow injection system for the determination of organophosphate and carbamate pesticides is described. A sensitive fluorescence probe was synthesized and used as the pH indicator to detect the inhibition of the enzyme acetylcholinesterase (AChE). The percentage inhibition of enzyme activity is correlated to the pesticide concentration. Several parameters influencing the performance of the system are discussed. The detection limits of 3.5, 50, 12 and 25 μg/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. A complete cycle of analysis, including incubation time, took 14 min. The detection system has been applied to the determination of carbofuran in spiked vegetable juices (Chinese cabbage and cole), achieving recovery values between 93.2 and 107% for Chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed.
KW - Acetylcholinesterase
KW - Carbamate pesticides
KW - Flow-injection analysis
KW - Fluorescence probe
KW - Organophosphate pesticides
KW - Vegetable juices
UR - https://www.scopus.com/pages/publications/4544289491
U2 - 10.1016/j.aca.2004.05.030
DO - 10.1016/j.aca.2004.05.030
M3 - 文章
AN - SCOPUS:4544289491
SN - 0003-2670
VL - 523
SP - 117
EP - 123
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -