TY - JOUR
T1 - Detection of sialic acid using boronic-acid-functionalized metal organic framework UiO-66-NH2@B(OH)2
AU - Yu, Huazheng
AU - Li, Yanhong
AU - Huang, Aisheng
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/9/1
Y1 - 2021/9/1
N2 - Sialic acid (SA) is a crucial component of glycoproteins and glycolipids on the cellular membrane, which is essential for maintaining the function of cell membranes, such as cell recognition and communication. Simultaneously, sialic acid plays a significant role in many physiological and pathological processes. Hence, it is urgent to develop a simple and sensitive strategy for determining sialic acid. In this work, a new metal-organic framework called UiO-66-NH2@B(OH)2 has been designed and synthesized for the recognition and detection of sialic acid. The boronic acid functional group in UiO-66-NH2@B(OH)2 can bind to a diol moiety of the glycerol side chain of sialic acid, which will attenuate or even quench the fluorescence of UiO-66-NH2@B(OH)2, thus opening a new road to detect sialic acid. Based on the measurement results, sialic acid can be quantitatively measured in a linear range of 0.05–2.5 mmoL/L with the UiO-66-NH2@B(OH)2 probe. The detection limit of sialic acid is as low as 0.025 mmol/L. Furthermore, the boronic-acid functionalized probe UiO-66-NH2@B(OH)2 displays high sensitivity and high selectivity to recognize the sialic acid in mouse serum samples. Therefore, the developed UiO-66-NH2@B(OH)2 can be used as a promising probe to identify and detect sialic acid in the practical application.
AB - Sialic acid (SA) is a crucial component of glycoproteins and glycolipids on the cellular membrane, which is essential for maintaining the function of cell membranes, such as cell recognition and communication. Simultaneously, sialic acid plays a significant role in many physiological and pathological processes. Hence, it is urgent to develop a simple and sensitive strategy for determining sialic acid. In this work, a new metal-organic framework called UiO-66-NH2@B(OH)2 has been designed and synthesized for the recognition and detection of sialic acid. The boronic acid functional group in UiO-66-NH2@B(OH)2 can bind to a diol moiety of the glycerol side chain of sialic acid, which will attenuate or even quench the fluorescence of UiO-66-NH2@B(OH)2, thus opening a new road to detect sialic acid. Based on the measurement results, sialic acid can be quantitatively measured in a linear range of 0.05–2.5 mmoL/L with the UiO-66-NH2@B(OH)2 probe. The detection limit of sialic acid is as low as 0.025 mmol/L. Furthermore, the boronic-acid functionalized probe UiO-66-NH2@B(OH)2 displays high sensitivity and high selectivity to recognize the sialic acid in mouse serum samples. Therefore, the developed UiO-66-NH2@B(OH)2 can be used as a promising probe to identify and detect sialic acid in the practical application.
KW - Fluorescent detection
KW - Fluorescent probe
KW - Metal-organic framework
KW - Sialic acid
KW - UiO-66-NH@B(OH)
UR - https://www.scopus.com/pages/publications/85104416539
U2 - 10.1016/j.talanta.2021.122434
DO - 10.1016/j.talanta.2021.122434
M3 - 文章
C2 - 34074419
AN - SCOPUS:85104416539
SN - 0039-9140
VL - 232
JO - Talanta
JF - Talanta
M1 - 122434
ER -