Detection of sialic acid using boronic-acid-functionalized metal organic framework UiO-66-NH₂@B(OH)₂

Sialic acid (SA) is a crucial component of glycoproteins and glycolipids on the cellular membrane, which is essential for maintaining the function of cell membranes, such as cell recognition and communication. Simultaneously, sialic acid plays a significant role in many physiological and pathological processes. Hence, it is urgent to develop a simple and sensitive strategy for determining sialic acid. In this work, a new metal-organic framework called UiO-66-NH₂@B(OH)₂ has been designed and synthesized for the recognition and detection of sialic acid. The boronic acid functional group in UiO-66-NH₂@B(OH)₂ can bind to a diol moiety of the glycerol side chain of sialic acid, which will attenuate or even quench the fluorescence of UiO-66-NH₂@B(OH)₂, thus opening a new road to detect sialic acid. Based on the measurement results, sialic acid can be quantitatively measured in a linear range of 0.05–2.5 mmoL/L with the UiO-66-NH₂@B(OH)₂ probe. The detection limit of sialic acid is as low as 0.025 mmol/L. Furthermore, the boronic-acid functionalized probe UiO-66-NH₂@B(OH)₂ displays high sensitivity and high selectivity to recognize the sialic acid in mouse serum samples. Therefore, the developed UiO-66-NH₂@B(OH)₂ can be used as a promising probe to identify and detect sialic acid in the practical application.