Detection of monoamine transmitters using high performance liquid chromatography with nanocrystalline Ce-doped lead dioxide deposited film chemically modified electrode

A novel nanocrystalline Ce-doped lead dioxide (PbO₂) deposited film modified glassy carbon electrode was prepared. The modified electrode was characterized by X-ray diffractometer (XRD) and scanning tunneling microscope (STM) techniques. The nanocrystalline Ce-doped PbO₂ deposited film modified electrode showed favorable electrocatalytic behavior toward the monoamine neurotransmitters with relatively high sensitivity and stability. Coupled to high-performance liquid chromatography (HPLC) with microdialysis, the modified electrode was utilized for electrochemical detection (ED) of these compounds. The method was successfully applied to assess the contents of the monoamines and their metabolites in the Parkinsonian patient's cerebrospinal fluid (CSF). The mechanism of Parkinsons disease was discussed elementarily.