TY - JOUR
T1 - Detection of Epstein–Barr virus (EBV)-encoded microRNAs in plasma of patients with nasopharyngeal carcinoma
AU - Gao, Wei
AU - Wong, Thian Sze
AU - Lv, Ke Xing
AU - Zhang, Min Juan
AU - Tsang, Raymond King Yin
AU - Chan, Jimmy Yu Wai
N1 - Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Background: Nasopharyngeal carcinoma (NPC) latently infected by Epstein–Barr virus (EBV) expresses 40 EBV BART microRNAs (miRNAs). Difference in diagnostic efficacy of these miRNAs on NPC detection was observed. Here, we performed a comprehensive evaluation on the efficacy of these miRNAs. Methods: Quantitative polymerase chain reaction was performed on plasma nucleic acid isolated from patients with NPC and noncancer donors. Results: For primary NPC, BART2-5P, BART6-3P, BART7-3P, BART7-5P, BART9-5P, BART11-3P, BART17-5P, and BART19-5P were significantly elevated. For recurrent NPC, plasma levels of BART2-3P, BART2-5P, BART5-3P, BART5-5P, BART6-3P, BART8-3P, BART9-5P, BART17-5P, BART19-3P, and BART20-3P were significantly increased. Area under curve (AUC) analysis showed that BART19-5P had the best performance to identify NPC which was serologically EBV DNA undetectable. For recurrent NPC, BART8-3P and BART10-3P had highest AUC value for identifying cancer in EBV DNA undetectable plasma. Conclusion: Our data supported the use of circulating EBV miRNAs in NPC and recurrent NPC detection.
AB - Background: Nasopharyngeal carcinoma (NPC) latently infected by Epstein–Barr virus (EBV) expresses 40 EBV BART microRNAs (miRNAs). Difference in diagnostic efficacy of these miRNAs on NPC detection was observed. Here, we performed a comprehensive evaluation on the efficacy of these miRNAs. Methods: Quantitative polymerase chain reaction was performed on plasma nucleic acid isolated from patients with NPC and noncancer donors. Results: For primary NPC, BART2-5P, BART6-3P, BART7-3P, BART7-5P, BART9-5P, BART11-3P, BART17-5P, and BART19-5P were significantly elevated. For recurrent NPC, plasma levels of BART2-3P, BART2-5P, BART5-3P, BART5-5P, BART6-3P, BART8-3P, BART9-5P, BART17-5P, BART19-3P, and BART20-3P were significantly increased. Area under curve (AUC) analysis showed that BART19-5P had the best performance to identify NPC which was serologically EBV DNA undetectable. For recurrent NPC, BART8-3P and BART10-3P had highest AUC value for identifying cancer in EBV DNA undetectable plasma. Conclusion: Our data supported the use of circulating EBV miRNAs in NPC and recurrent NPC detection.
KW - EBV DNA
KW - EBV-encoded microRNA
KW - Epstein–Barr virus
KW - circulating nucleic acid
KW - nasopharyngeal carcinoma
UR - https://www.scopus.com/pages/publications/85058396457
U2 - 10.1002/hed.25544
DO - 10.1002/hed.25544
M3 - 文章
C2 - 30548946
AN - SCOPUS:85058396457
SN - 1043-3074
VL - 41
SP - 780
EP - 792
JO - Head and Neck
JF - Head and Neck
IS - 3
ER -
