Dendritic Mesoporous Silica Nanoparticles with Abundant Ti4+ for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity

Yayun Hong; Yating Yao; Hongli Zhao; Qianying Sheng; Mingliang Ye; Chengzhong Yu; Minbo Lan

doi:10.1021/acs.analchem.8b01369

Dendritic Mesoporous Silica Nanoparticles with Abundant Ti⁴⁺ for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity

Yayun Hong
, Yating Yao
, Hongli Zhao^*
, Qianying Sheng
, Mingliang Ye
, Chengzhong Yu
, Minbo Lan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti⁴⁺ (denoted DMSNs@PDA-Ti⁴⁺) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti⁴⁺ with a high surface area (362 m² g^-1), a large pore volume (1.37 cm³ g^-1), and a high amount of chelated Ti⁴⁺ (75 μg mg^-1). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO₂@PDA-Ti⁴⁺) and commercial TiO₂, DMSNs@PDA-Ti⁴⁺ showed better selectivity and a lower detection limit (0.2 fmol/μL). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95%) enabled by DMSNs@PDA-Ti⁴⁺, better than those in previous reports.

Original language	English
Pages (from-to)	7617-7625
Number of pages	9
Journal	Analytical Chemistry
Volume	90
Issue number	12
DOIs	https://doi.org/10.1021/acs.analchem.8b01369
State	Published - 19 Jun 2018
Externally published	Yes

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@article{aebd8c5aa7d540fe8bb8e3fe09873bd3,

title = "Dendritic Mesoporous Silica Nanoparticles with Abundant Ti4+ for Phosphopeptide Enrichment from Cancer Cells with 96\% Specificity",

abstract = "Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti4+ (denoted DMSNs@PDA-Ti4+) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti4+ with a high surface area (362 m2 g-1), a large pore volume (1.37 cm3 g-1), and a high amount of chelated Ti4+ (75 μg mg-1). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO2@PDA-Ti4+) and commercial TiO2, DMSNs@PDA-Ti4+ showed better selectivity and a lower detection limit (0.2 fmol/μL). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95\%) enabled by DMSNs@PDA-Ti4+, better than those in previous reports.",

author = "Yayun Hong and Yating Yao and Hongli Zhao and Qianying Sheng and Mingliang Ye and Chengzhong Yu and Minbo Lan",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = jun,

day = "19",

doi = "10.1021/acs.analchem.8b01369",

language = "英语",

volume = "90",

pages = "7617--7625",

journal = "Analytical Chemistry",

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TY - JOUR

T1 - Dendritic Mesoporous Silica Nanoparticles with Abundant Ti4+ for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity

AU - Hong, Yayun

AU - Yao, Yating

AU - Zhao, Hongli

AU - Sheng, Qianying

AU - Ye, Mingliang

AU - Yu, Chengzhong

AU - Lan, Minbo

PY - 2018/6/19

Y1 - 2018/6/19

N2 - Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti4+ (denoted DMSNs@PDA-Ti4+) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti4+ with a high surface area (362 m2 g-1), a large pore volume (1.37 cm3 g-1), and a high amount of chelated Ti4+ (75 μg mg-1). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO2@PDA-Ti4+) and commercial TiO2, DMSNs@PDA-Ti4+ showed better selectivity and a lower detection limit (0.2 fmol/μL). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95%) enabled by DMSNs@PDA-Ti4+, better than those in previous reports.

AB - Selective enrichment and sensitive detection of phosphopeptides are of great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine and chelated Ti4+ (denoted DMSNs@PDA-Ti4+) were developed to improve the enrichment selectivity of phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti4+ with a high surface area (362 m2 g-1), a large pore volume (1.37 cm3 g-1), and a high amount of chelated Ti4+ (75 μg mg-1). Compared with conventional mesoporous silica-based materials with the same functionalization (denoted mSiO2@PDA-Ti4+) and commercial TiO2, DMSNs@PDA-Ti4+ showed better selectivity and a lower detection limit (0.2 fmol/μL). Moreover, 2422 unique phosphopeptides were identified from HeLa cell extracts with a high specificity (>95%) enabled by DMSNs@PDA-Ti4+, better than those in previous reports.

UR - https://www.scopus.com/pages/publications/85047720647

U2 - 10.1021/acs.analchem.8b01369

DO - 10.1021/acs.analchem.8b01369

M3 - 文章

C2 - 29799184

AN - SCOPUS:85047720647

SN - 0003-2700

VL - 90

SP - 7617

EP - 7625

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 12

ER -

Dendritic Mesoporous Silica Nanoparticles with Abundant Ti⁴⁺ for Phosphopeptide Enrichment from Cancer Cells with 96% Specificity

Abstract

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