Abstract
AIM: To construct DNA vaccine expression plasmid containing the chimeric gene gag-gp120 of HIV-1.
METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag-gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT-PCR and analyzed by Dot-ELISA.
RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot-ELISA detection showed that the target gene was expressed in Hela cells.
CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag-gp120 was successfully constructed, which lays the foundation for preparing DNA vaccine against HIV-1.
| Original language | English |
|---|---|
| Pages (from-to) | 341-342 |
| Number of pages | 2 |
| Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology |
| Volume | 19 |
| Issue number | 4 |
| State | Published - 1 Jul 2003 |
| Externally published | Yes |
